Application of Morphological Analysis and DNA Barcode in Determination of The Porcelain Crab Species (Pisidia sp.) from The Tunda Island, Banten

JURNAL SUMBERDAYA AKUATIK INDOPASIFIK

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Title Application of Morphological Analysis and DNA Barcode in Determination of The Porcelain Crab Species (Pisidia sp.) from The Tunda Island, Banten
APLIKASI ANALISA MORFOLOGI DAN DNA BARCODING PADA PENENTUAN JENIS KEPITING PORCELAIN (PISIDIA Sp.) YANG BERASAL DARI PULAU TUNDA, BANTEN
 
Creator Triandiza, Teddy
Maddupa, Hawis
 
Description The phenomenon of marine cryptic species is frequently found in altered ecosystem such as dead corals habitat. Dead corals as habitat can be used by marine fauna like crustacean from family of porcellanidae. This study was aimed to identify and clarify the species of porcelain crab from Tundai sland, Banten province by applying the morphological analysis and DNA barcoding technique. The result of morphological analysis showed that decapod samples were crabs of the genus Pisidia, family of Porcellanidae with specific morphological characteristics were the ratio of carapace size is longer than tbroad, have long antennae which can be moved, serrated anterior rostrum, chelae rather flattish and unequal size of arms. While based on molecular barcode analysis by using COI, samples were identified as Anomura sp. with 94% of similarities. The difference of the result was suspected due to the lack of suitable genetic data on genbank.
Fenomena cryptic species (hampir mirip) pada biota akuatik sering ditemukan pada ekosistem yang mengalami perubahan, seperti karang mati.  Karang mati menjadi habitat yang masih dapat dimanfaatkan biota laut salah satunya kepiting decapoda familia Porcellanidae.. Penelitian ini bertujuan untuk mengidentifikasi dan memastikan jenis dari kepiting porcelain yang berasal dari Pulau Tunda, Banten dengan mengaplikasi analisa mororfologi dan teknik DNA barcoding. Hasil analisa morfologi menunjukkan bahwa sampel decapoda yang di analisis merupakan kepiting dari genus Pisidia familia Porcellanidae dengan karekteristik morfologi rasio ukuran karapas lebih panjang dari pada lebar, antena panjang dan bisa digerakkan, bagian rostrum anterior bergerigi, capit agak pipih dan ukuran lengan tidak sama besar. Sedangkan berdasarkan analisis barcode secara molekuler menggunakan COI, maka didapatkan hasil Anomura sp. dengan persentase kesamaan identifikasi 94%. Perbedaan tersebut di duga karena pada genbank belum terdapat data genetik yang sesuai.
 
Publisher Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua
 
Date 2018-11-19
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://ejournalfpikunipa.ac.id/index.php/JSAI/article/view/51
10.30862/jsai-fpik-unipa.2018.Vol.2.No.2.51
 
Source JURNAL SUMBERDAYA AKUATIK INDOPASIFIK; Volume 2, Number 2, November 2018; 81-90
JURNAL SUMBERDAYA AKUATIK INDOPASIFIK; Volume 2, Number 2, November 2018; 81-90
2550-0929
2550-1232
 
Language eng
 
Relation https://ejournalfpikunipa.ac.id/index.php/JSAI/article/view/51/31
 
Rights Copyright (c) 2018 JURNAL SUMBERDAYA AKUATIK INDOPASIFIK
 

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