Expressions of stemness markers in keloid tissue

Medical Journal of Indonesia

View Publication Info
 
 
Field Value
 
Title Expressions of stemness markers in keloid tissue
 
Creator Ningsih, Sri S.; Master of Biomedical Sciences, Faculty of Medicine, University of Indonesia, Jakarta
Sari, Dewi H.; Master of Biomedical Sciences, Faculty of Medicine, University of Indonesia, Jakarta
Antarianto, Radiana D.; Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta
Hardiany, Novi S.; Department of Biochemistry and Molecular Biology, Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine, Universitas Indonesia, Jakarta
Sadikin, Mohamad; Department of Biochemistry and Molecular Biology, Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine, Universitas Indonesia, Jakarta
Wanandi, Septelia I.; Department of Biochemistry and Molecular Biology, Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine, Universitas Indonesia, Jakarta
Jusman, Sri W.A.; Department of Biochemistry and Molecular Biology, Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine, Universitas Indonesia, Jakarta
 
Subject ALDH1; keloid; Oct-4; stemness
 
Description Background: Keloid is an abnormal wound healing process that extends beyond the site of injury. Keloid and tumor’s shared similarity of recurrence suggesting a shared underlying mechanism that involves stemness. Octamer-binding transcription factor-4 (Oct-4) and aldehyde dehydrogenase-1 (ALDH1) are stem cell stemness markers. This study aimed to analyze Oct-4 and ALDH1 expressions in keloid tissues.Methods: Samples were obtained from keloid tissue excisions from three keloid patients and post-circumcision preputial skin from three healthy donors (normal control) in accordance with the local ethical committee regulation. Total RNA was isolated using TriPure Isolation kit (Ameritech), and expressions of Oct4 and ALDH1 mRNA in keloid and preputial skin were determined by quantitative reverse transcription–polymerase chain reaction (qRT-PCR) using Livak method.Results: The qRT-PCR analysis revealed the expressions of Oct4 and ALDH1 in keloid and preputial skin tissues. Keloid tissues exhibited lower expression levels of Oct-4 and ALDH1 than the preputial skin. The difference was statistically insignificant.Conclusion: Keloid tissues express Oct-4 and ALDH1 as stemness markers, and the stemness characteristics of keloid might be similar to a normal skin.
 
Publisher Faculty of Medicine Universitas Indonesia
 
Contributor Direktorat Riset dan Pengabdian Masyarakat UI which gives us funding to conduct this research through Hibah Publikasi Ilmiah Terindeks Tugas Akhir (PITTA).
 
Date 2018-10-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://mji.ui.ac.id/journal/index.php/mji/article/view/1920
10.13181/mji.v27i3.1920
 
Source Medical Journal of Indonesia; Vol 27, No 3 (2018): September; 145-9
2252-8083
0853-1773
10.13181/mji.v27i3
 
Language eng
 
Relation http://mji.ui.ac.id/journal/index.php/mji/article/view/1920/1240
 
Rights Copyright (c) 2018 Sri S. Ningsih, Dewi H. Sari, Radiana D. Antarianto, Novi S. Hardiany, Mohamad Sadikin, Septelia I. Wanandi, Sri W.A. Jusman
http://creativecommons.org/licenses/by-nc/4.0
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library