Limonene and BEZ 235 induce apoptosis in COLO-320 and HCT-116 colon cancer cells

International Journal of Drug Delivery

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Title Limonene and BEZ 235 induce apoptosis in COLO-320 and HCT-116 colon cancer cells
 
Creator Yakkanti, Raja Ratna Reddy; Sugen Life Sciences Pvt Ltd., Tirupati, A P, India. Manipal University, Manipal, Karnataka, India. Palamur Biosciences Pvt Ltd., Mahabubnagar, Telangana, India.
Singh, Manisha; S.V.S Medical College, Mahabubnagar, Telangana, India
Kabra, Vasanti; S.V.S Medical College, Mahabubnagar, Telangana, India
Sekhar, P. Chandra; Manipal University, Manipal, Karnataka, India. Palamur Biosciences Pvt Ltd., Mahabubnagar, Telangana, India.
SreejaVamsi, K.; S.V.S Medical College, Mahabubnagar, Telangana, India
Reddy, B. Ramachandra; S.V.S Medical College, Mahabubnagar, Telangana, India
Ramamoorthy, S; Palamur Biosciences Pvt Ltd., Mahabubnagar, Telangana, India.
Reddy, C. Damodar; Sugen Life Sciences Pvt Ltd., Tirupati, A P, India.
 
Subject Cancer biology, new drug development
Colorectal cancer, limonene, BEZ, apoptosis, caspases.
 
Description Deregulated apoptosis is the hall mark of many cancers, therefore every defect in apoptosis pathway could be a potential target for cancer treatment.The anticancer mechanism of limonene could be multifactorial. However, induction of apoptosis in cancer cells is proposed as the predominant mechanism in several of preclinical studies. Therefore, we determined to investigate the role of apoptosis in the anticancer activity of limonene and BEZ235 combination in COLO-320 and HCT-116 colon cancer cells. Cells after treatments were assessed for apoptosis by DAPI staining for fluorescent microscopic examination of apoptotic cells, estimation of caspases activities, Bcl-2 family proteins in addition to cell cycle analysis by flowcytometry. Results show that both drugs induced apoptosis as demonstrated by increased caspases activity, significant alterations in pro and anti-apoptotic proteins of Bcl-2 family in promoting apoptosis and cell cycle arrest at G1 phase. Over all, it is indicated that limonene and BEZ exerted anticancer activity is mediated through induction of apoptosis involving mitochondria mediated intrinsic death pathway in the selected CRC cells.
 
Publisher Advanced Research Journals
 
Contributor Sugen Life Sciences Pvt Ltd, Palamur Biosciences Pvt Ltd, S.V.S Medical College
 
Date 2018-08-25
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://www.arjournals.org/index.php/ijdd/article/view/2240
10.5138/09750215.2240
 
Source International Journal of Drug Delivery; Vol 10, No 2 (2018): International Journal of Drug Delivery; 19-24
0975-0215
 
Language eng
 
Relation http://www.arjournals.org/index.php/ijdd/article/view/2240/pdf
 
Rights Copyright (c) 2018 raja ratna reddy yakkanti
http://creativecommons.org/licenses/by-nc-nd/4.0
 

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