Curcumin induces apoptosis in trophoblast model cell line

Medical Journal of Indonesia

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Title Curcumin induces apoptosis in trophoblast model cell line
 
Creator Nurseta, Tatit; Department of Obstetric and Gynecology, Faculty of Medicine, Universitas Brawijaya, Malang
Irwanto, Yahya; Division of Oncology Gynecology, Department of Obstetric and Gynaecology, Saiful Anwar Hospital, Faculty of Medicine, Universitas Brawijaya, Malang
Wiyasa, I W.A.; Division of Fertility, Endocrinology and Reproduction, Obstetric and Gynaecology Laboratory, Saiful Anwar Hospital, Faculty of Medicine, Universitas Brawijaya, Malang
Rahajeng, Rahajeng; Division of Urogynecology, Obstetric and Ginaecology Laboratory, Saiful Anwar Hospital, Faculty of Medicine, Universitas Brawijaya, Malang
Imelda, Imelda; Resident of Obstetric and Gynaecology, Saiful Anwar Hospital, Faculty of Medicine, Universitas Brawijaya, Malang
Ratnawati, Putu A.R.; Resident of Obstetric and Gynaecology, Saiful Anwar Hospital, Faculty of Medicine, Universitas Brawijaya, Malang
 
Subject apoptosis; curcumin; eNOS; hydatidiform mole; trophoblast
 
Description Background: Several studies have reported that curcumin exerts chemopreventive effects in various type of cancers, through several mechanisms, however, the effect of curcumin on carcinogenesis in patients with hydatidiform mole has not yet been investigated. This study was conducted to evaluate the effect of curcumin on apoptosis, proliferation, and nuclear translocation of endothelial nitricoxide synthase in trophoblast cells induced by estradiol in complete hydatidiform mole (CHM).Methods: In this in vitro study, trophoblast cells were divided into six groups, the control group (trophoblast cells were exposed to 100 pg/mL of 17-β estradiol) and the treatment group (trophoblast cells were exposed to 100 pg/mL of 17-β estradiol in the presence of curcumin with doses: 50, 100, 200, 400, and 800 µM). At the end of study, the cell proliferation was analyzed using MTT assay and apoptosis with TUNEL test in each group thropoblast cell. eNOS translocation was assayed using confocal laser scanning microscopy at the various dose of curcumin.Results: Curcumin at the doses of 200, 400, and 800 µM significantly decreased the proliferation and increased the apoptotic index in curcumin-treated group compared to those in the control group (p<0.05). All doses of curcumin treatment significantly decreased the nuclear eNOS expression compared to that in the control group. The three highest doses of curcumin increased cytoplasmic eNOS expression compared to that in control group.Conclusion: Curcumin inhibits the proliferation and modulates the apoptosis of trophoblast cells induced by estradiol in CHM involvement.
 
Publisher Faculty of Medicine Universitas Indonesia
 
Contributor
 
Date 2018-09-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://mji.ui.ac.id/journal/index.php/mji/article/view/1821
10.13181/mji.v27i2.1821
 
Source Medical Journal of Indonesia; Vol 27, No 2 (2018): June; 87–93
2252-8083
0853-1773
10.13181/mji.v27i2
 
Language eng
 
Relation http://mji.ui.ac.id/journal/index.php/mji/article/view/1821/1231
 
Rights Copyright (c) 2018 Tatit Nurseta, Yahya Irwanto, I W.A. Wiyasa, Rahajeng Rahajeng, Imelda Imelda, Putu A.R. Ratnawati
http://creativecommons.org/licenses/by-nc/4.0
 

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