EFFECT OF ETHANOL EXTRACT OF Eleutherine bulbosa (MILL.) URB ON ANAEROBIC BACTERIAL Prophyromonas gingivalis IN VITRO

Journal of Tropical Pharmacy and Chemistry

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Title EFFECT OF ETHANOL EXTRACT OF Eleutherine bulbosa (MILL.) URB ON ANAEROBIC BACTERIAL Prophyromonas gingivalis IN VITRO
 
Creator Azizah, Siti Nur
Yani, Sinar
Ismail, Sjarif
Masyudhi, Masyudhi
Sawitri, Endang
 
Description Periodontitis is an inflamation of supporting tissues on teeth that became the main cause of Prophyromonas gingivalis (P. gingivalis) bacteria. Treatment of periodontitis is by giving antibacterial agent therapy. Plants that have antibacterial effects one are Eleutherine bulbosa (UEB) bulbs from the Iridaceae family. This plant has been used for ethnobotany boils drugs and has been known to have antibacterial activity toward against intestinal pathogen bacteria that is anaerobic Gram Negative, but still unknown its activity against on oral pathogenic bacteria such as P. gingivalis. The purpose of this study was to know the effect of UEB extract on the growth of P. gingivalis bacteria as in vitro. UEB is taken from agriculture center in Samarinda city. UEB Simplisia was tested for antibacterial activity using Kirby-Bauer disc diffusion method on BHI-A media supplemented with vitamin K and hemin. The bacteria which used were P. gingivalis ATCC® 33277. Blank disc 6 mm with nine concentrations attached to BHI-A medium, incubated for 24 hours at 37 °C in an anaerobic atmosphere, then sprayed MTT (3-[4,5-Dimethylthiazole-2-yl]-2,5-diphenylttetrazoliumbromide) reagent and read a few moments later. For comparison, then used Chlorhexidine gluconate (CHX) 2 mg/ml. The statistical analysis with t-test and there was significant different if  p<0.05. The result showed that the higher concentration of UEB ethanol extract will increase the growth inhibition zone of P. gingivalis bacteria and reach maximum at 10 mg/ml concentration. The result of t-test showed no difference significant of P. gingivalis bacterial inhibition zone in the treatment group of 7.5 mg/ml and 10 mg/ml concentration on CHX group. This study proves UEB ethanol extract can inhibit the growth of P. gingivalis bacteria.  
 
Publisher Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117
 
Date 2018-07-26
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/151
10.25026/jtpc.v4i3.151
 
Source Journal of Tropical Pharmacy and Chemistry; Vol 4 No 3 (2018): Journal of Tropical Pharmacy and Chemistry; 128-135
2407-6090
2087-7099
10.25026/jtpc.v4i3
 
Language eng
 
Relation https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/151/154
 
Rights Copyright (c) 2018 Journal of Tropical Pharmacy and Chemistry
http://creativecommons.org/licenses/by-nc/4.0
 

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