PENGARUH JENIS DAN LAMA PERENDAMAN BAHAN STERILAN TERHADAP EKSPLAN ANTER GAMBIR (Uncaria gambir (Hunter) Roxb.)

Jurnal BiBieT

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Title PENGARUH JENIS DAN LAMA PERENDAMAN BAHAN STERILAN TERHADAP EKSPLAN ANTER GAMBIR (Uncaria gambir (Hunter) Roxb.)
 
Creator Resigia, Elara; Universitas Tamansiswa
 
Subject Kata kunci : Bunga gambir; Kultur in vitro;Sterilisasi
 
Description Penelitian ini bertujuan untuk mendapatkan kombinasi jenis sterilan dan lama perendaman dari bahan sterilan tersebut. Rancangan yang digunakan pada penelitian ini adalah Rancangan Acak Lengkap. Data yang diperoleh dianalisis secara deskriptif Hasil penelitian menunjukkan sterilisasi bunga gambir terbaik adalah bunga gambir yang dijadikan sebagai eksplan disemprot dengan fungisida dan bakterisida pada sore hari dan diisolasi sebelum bunga diambil pada besok pagi, bunga yang diambil di lapangan, kemudian dibilas dengan air mengalir dan direndam dengan bayclin 10 % selama 1 menit serta dibilas dengan aquades steril sebanyak tiga kali, terakhir bunga direndam dengan asam askorbit 0,02 % selama 5 menit.This study aims to obtain a combination of sterilan and immersion of the sterilan. The design used in this study is Completely Randomized Design. The data obtained were analyzed descriptively. The results showed that the best gambir flower sterilization was used as explant spray with fungicide and bactericide in the afternoon and isolated before the flowers were taken tomorrow morning, the flower taken in the field, then rinsed with running water and soaked With 10% bayclin for 1 minute and rinsed with sterile aquades three times, the last flower was soaked with 0.02% ascorbic acid for 5 minutes. 
 
Publisher Lembaga Layanan Pendidikan Tinggi (LLDIKTI) Wilayah X
 
Contributor
 
Date 2017-10-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://ejournal.kopertis10.or.id/index.php/bibiet/article/view/2802
10.22216/jbbt.v2i2.2802
 
Source Jurnal BiBieT; Vol 2, No 2 (2017): BIBIET; 44-48
Jurnal BiBiet; Vol 2, No 2 (2017): BIBIET; 44-48
2502-0951
10.22216/jbbt.v2i2
 
Language eng
 
Relation http://ejournal.kopertis10.or.id/index.php/bibiet/article/view/2802/995
 
Rights Copyright (c) 2017 Elara Resigia
http://creativecommons.org/licenses/by-sa/4.0
 

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