Preparation of cyclodextrin nanoparticles and evaluation of its effect on the capacitation of bovine spermatozoa used in the in vitro fertilization

International Journal of Drug Delivery

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Title Preparation of cyclodextrin nanoparticles and evaluation of its effect on the capacitation of bovine spermatozoa used in the in vitro fertilization
 
Creator Salem, Heba F; Pharmaceutics and Industrial pharmacy Department, Faculty of Pharmacy, Beni-Suef University, Egypt
Raslan, Mai; Biotechnology Department, Faculty of Post graduate for advanced sciences, Beni-Suef University, Egypt
Suliman, Hanaa; Biotechnology Department, Faculty of Post graduate for advanced sciences, Beni-Suef University, Egypt
Essam, Tamer; Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Egypt.
Abd-Allah, Saber; Theriogenology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt
 
Subject Pharmaceutical Sciences; Pharmaceutics; Drug Delivery
Capacitaion; cyclodextrin; nanoparticles; In Vitro Fertilization; spermatozoa.
 
Description This study was conducted to produce nanosized cyclodextrin (NCD) and assess its effect on bovine spermatozoa during In vitro fertilization (IVF) to optimize the capacitation media for successful IVF. Therefore, Four cyclodextrin formulations were prepared and characterized. Data analysis revealed the best formula (F2) showed a smallest particle size (15 nm), zeta potential (-37 mv), and higher yield percentages (95%) was selected for spem capacitation. Motile spermatozoa were separated from frozen-thawed semen by a swim-up procedure and capacitated in IVF-TALP medium with different formulae of NCD or CD or without treatments (control) and incubated for 3hours(hr) at 38°C and evaluated every one (hr) interval. Data analysis revealed that the formulation of cyclodextrin nanoparticles (F2) after (2hr) incubation in the media gave best effect on sperm capacitation and acrosme reaction (AR) and effect of sperm treated with NCD on fertilization rate was evaluated. The results showed that the proportion of Oocytes fertilized was increased significantly in F2 (60%) than in the control (35%), and cyclodextrin group (50%) groups (p<0.05). It could be inferred from this investigation that cyclodextrin nanoparticles can be used for biomedical interventions in bovine spermatozoa. NCD improve sperm motility, viability, and (AR), also fertilization rate of sperm treated with NCD increase. So NCD gave positive effect on sperm functions during IVF. 
 
Publisher Advanced Research Journals
 
Contributor
 
Date 2018-01-23
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://www.arjournals.org/index.php/ijdd/article/view/2196
10.5138/09750215.2196
 
Source International Journal of Drug Delivery; Vol 9, No 4 (2017): International Journal of Drug Delivery; 112-118
0975-0215
 
Language eng
 
Relation http://www.arjournals.org/index.php/ijdd/article/view/2196/pdf
 
Rights Copyright (c) 2017 hanaa suliman, Heba Salem, Saber Abd-Allah, Mai Ralan, Tamer Essam
http://creativecommons.org/licenses/by-nc-nd/4.0
 

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