Preparation of progesterone nanoparticles and evaluation of its effect on the capacitation of Bovine spermatozoa used in the in Vitro Fertilization

International Journal of Drug Delivery

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Title Preparation of progesterone nanoparticles and evaluation of its effect on the capacitation of Bovine spermatozoa used in the in Vitro Fertilization
 
Creator Abd-Allah, Saber; Theriogenology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt
Ralan, Mai; Biotechnology Department, Faculty of Post graduate for Advanced Sciences, Beni-Suef University, Egypt
Suliman, Hanaa; Biotechnology Department, Faculty of Post graduate for advanced sciences, Beni-Suef University, Egypt
Essam, Tamer; 3Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Egypt.
Salem, Heba F; Pharmaceutics and Industrial pharmacy Department, Faculty of Pharmacy, Beni-Suef University, Egypt
 
Subject Pharmaceutical Sciences; Pharmaceutics; Drug Delivery
Capacitation; In vitro fertilization; Nanoparticles; Progesterone; Spermatozoa.
 
Description Progesterone (P) has been reported to affect several sperm functions especially capacitation and acrosome reaction. The main problem of (P) is its low aqueous solubility. So formulation of progesterone nanoparticles (PN) will enhance its solubility. This study was conducted to produce nanosized progesterone (NP) and assess its biocompatibility. Therefore, nine progesterone formulations were prepared and characterized. Data analysis revealed only one formula of P showed nanosized particle (1-100 nm) with an average particle size (95±5 nm), and spherical shape as seen by Transmission Electron Microscope(TEM). Motile spermatozoa were separated from frozen-thawed semen by a swim-up procedure and capacitated in IVF-TALP medium with NP or P or without treatments (control) and incubated for 3h at 38°C and evaluated every 1 hour (h) interval. Ovarian oocytes were matured and fertilized in vitro with frozen-thawed bull sperm capacitated in vitro with NP or P or control (without NP, P) and incubated at 39C in 5% CO2 incubator for 24h and then examined for evidence of fertilization. In conclusion, this study demonstrates that nanosized progesterone is highly efficient for sperm capacitation. In addition to the use of nanosized progesterone in sperm capacitation produces more fertilized oocytes than the progesterone after In Vitro Fertilization (IVF).
 
Publisher Advanced Research Journals
 
Contributor
 
Date 2018-01-23
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://www.arjournals.org/index.php/ijdd/article/view/2224
10.5138/09750215.2224
 
Source International Journal of Drug Delivery; Vol 9, No 4 (2017): International Journal of Drug Delivery; 125-131
0975-0215
 
Language eng
 
Relation http://www.arjournals.org/index.php/ijdd/article/view/2224/pdf
 
Rights Copyright (c) 2017 Saber Abd-Allah, Mai Ralan, hanaa suliman, Tamer Essam, Heba Salem
http://creativecommons.org/licenses/by-nc-nd/4.0
 

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