Identifikasi Ikan Genus Mystus Dengan Pendekatan Genetik

JURNAL SUMBERDAYA AKUATIK INDOPASIFIK

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Title Identifikasi Ikan Genus Mystus Dengan Pendekatan Genetik
Identification of Mystus Fish With Genetic Approach
 
Creator Pramono, Taufik Budhi
Arfiati, Diana Arfiati
Widodo, Maheno Sri
Yanuhar, Uun Yanuhar
 
Description Ikan-ikan dari Famili Bagridae di Indonesia mencapai 60 jenis dan salah satunya dari genus Msytus. Metode yang digunakan untuk identifikasi ikan yang sangat cepat dan akurat sangat diperlukan.  DNA barcoding adalah teknik identifikasi baru dengan pendekatan molekular. Gen CO1 diamplifikasi dan produk PCR disekuensing serta dianalisis dengan menggunakan software bioinformatika.  Pengeditan hasil sekuensing dan penentuan komposisi nukleotida dianalisis dengan software Mega5. Urutan DNA disejajarkan dengan ClustalW vers. 1.4. Sekuens dibandingkan dengan data GenBank menggunakan BLAST (Basic Local Aligment Search Tools) dan BOLDSystems.  Pohon filogenetik dibuat dengan menggunakan metode Neighbor_Joining. Salah satu contoh identifikasi ikan dari genus Mystus yang telah dikonfirmasi adalah Mystus nigriceps menjadi Mystus singaringan.
The fish of the Bagridae Family in Indonesia reach  until 60 species and one of them is from the genus Msytus.  The method is used for fast and accurate species identification was needed. DNA barcoding was a new identification method with molecularly approach. The CO1 gene was amplified and PCR products were sequenced and analyzed using bioinformatics software. Editing of sequencing results and determining the nucleotide composition were analyzed with Mega5 software. The DNA sequence was aligned with ClustalW vers. 1.4. Sequences are compared with GenBank data using BLAST (Basic Local Aligment Search Tools) and BOLDSystems. Phylogenetic tree was made using Neighbor_Joining method. One example of the identification of fish from the confirmed Mystus genus is Mystus nigriceps being the Mystus singaringan.
 
Publisher Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua
 
Date 2017-12-03
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://ejournalfpikunipa.ac.id/index.php/JSAI/article/view/34
10.30862/jsai-fpik-unipa.2017.Vol.1.No.2.34
 
Source JURNAL SUMBERDAYA AKUATIK INDOPASIFIK; Volume 1, Number 2, November 2017; 123-132
JURNAL SUMBERDAYA AKUATIK INDOPASIFIK; Volume 1, Number 2, November 2017; 123-132
2550-0929
2550-1232
 
Language ind
 
Relation https://ejournalfpikunipa.ac.id/index.php/JSAI/article/view/34/15
 
Rights Copyright (c) 2017 JURNAL SUMBERDAYA AKUATIK INDOPASIFIK
 

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