Application of Artificial Seeds as A Method of In Vitro Conservation of Orchids: Encapsulación de embriones

Revista Mutis

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Title Application of Artificial Seeds as A Method of In Vitro Conservation of Orchids: Encapsulación de embriones
Aplicación de semillas artificiales como método de conservación in vitro de orquídeas: Encapsulación de embriones
 
Creator Cárdenas Guarín, Jurghen Hernando
Sigarroa Rieche, Alina Katil
Salazar Mercado, Seir Antonio
 
Subject alginate
seed preservation
gelling agents
Orchidaceae
synthetic seeds
Alginato
Conservación de semillas
Gelificantes
Orchidaceae
Semillas Sintéticas
 
Description  
The ex-situ conservation techniques of plant germplasm have become essential tools for the preservation of biodiversity in the world. Orchids are known to be one of the most affected families by the problem of biodiversity loss, and great efforts are required to genetically secure the germplasm of these species. Over the years, plant tissue culture has become the most practical and effective method for the conservation and propagation of the Orchidaceae family, due to the large amount of material that can be extracted from a limited number of plants. However, it is a well-known fact that the shelf life of orchid seeds is short. Biotechnological development has allowed the implementation of techniques such as the encapsulation of plant embryos, which has obtained effective and favorable results for the conservation of orchid germplasm. In this work, some of the most significant advances in the technique of artificial seeds are reviewed and their main differences and similarities with other plant preservation techniques used for the conservation of orchids are highlighted. It is thus demonstrated that embryo encapsulation techniques represent a great advance for the conservation of orchids in the short- and medium-term, and the optimization of the protocols will allow their use in different laboratories in the region and the country. It will also contribute to the standardization of the technique and its implementation in different varieties of orchids, obtaining a significant impact on the conservation of germplasm of this family.
Las técnicas de conservación ex situ de germoplasma vegetal se han convertido en herramientas imprescindibles para la preservación de la biodiversidad en el mundo. Las orquídeas son conocidas por ser una de las familias más afectadas por este problema, y se requieren de grandes esfuerzos para asegurar genéticamente el germoplasma de estas especies. Con el paso de los años, el cultivo de tejidos vegetales se ha establecido como el método más práctico y efectivo para la conservación y propagación de la familia Orchidaceae, debido a la gran cantidad de material que se puede disponer de un número limitado de plantas. Sin embargo, es bien conocido el corto tiempo de vida en almacenamiento que presentan las semillas de las orquídeas. Desde el desarrollo biotecnológico se han implementado técnicas como la encapsulación de embriones vegetales, la cual ha obtenido resultados efectivos y favorables para la conservación de germoplasma de las orquídeas. En la presente revisión se hace una recopilación de algunos de los avances más significativos en la técnica de semillas artificiales, resaltando sus principales diferencias y similitudes con otras técnicas de preservación vegetal utilizadas para la conservación de orquídeas. Se demuestra así que las técnicas de encapsulación de embriones representan un gran avance para la conservación de orquídeas a corto y mediano plazo, y la optimización de los protocolos permitirá su uso en diferentes laboratorios de la región y el país, y contribuirá a la estandarización de la técnica y su implementación en distintas variedades de orquídeas, obteniendo un impacto significativo en la conservación de germoplasma de esta familia.
 
Publisher Universidad de Bogotá Jorge Tadeo Lozano
 
Date 2022-01-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://revistas.utadeo.edu.co/index.php/mutis/article/view/Aplicacion-semillas-artificiales-metodo-conservacion-in-vitro-orquideas
10.21789/22561498.1818
 
Source Revista Mutis; Vol. 12 Núm. 1 (2022)
Revista Mutis; Vol. 12 No. 1 (2022)
2256-1498
 
Language spa
 
Relation https://revistas.utadeo.edu.co/index.php/mutis/article/view/Aplicacion-semillas-artificiales-metodo-conservacion-in-vitro-orquideas/1856
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