IDENTIFIKASI VIRUS PENYAKIT JEMBRANA PADA SAPI BALI MENGGUNAKAN POLYMERASE CHAIN REACTION (PCR) DI PROVINSI KEPULAUAN BANGKA BELITUNG

Berita Biologi

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Title IDENTIFIKASI VIRUS PENYAKIT JEMBRANA PADA SAPI BALI MENGGUNAKAN POLYMERASE CHAIN REACTION (PCR) DI PROVINSI KEPULAUAN BANGKA BELITUNG
 
Creator Irwanto, Riko
Adi Sulistyo, Correy Wahyu
Ari, Ari
Jeffri Irawan, Aditya
 
Subject Bali cattle, Jembrana, PCR.
 
Description Bali cattle is one of the typical Indonesian livestock that is widely cultivated because of its high reproductive and adaptation capabilities to the environment. However, behind these advantages, bali cattle have a weakness, namely they are susceptible to the jembrana virus which can cause jembrana disease. This disease will cause a decrease in the immune system of bali cattle so that they are susceptible to other diseases that will lead to the death of the cattle. The method used in the identification of the Jembrana disease virus is the Polymerase Chain Reaction-Reverse Transcription (PCR-RT) technique. Identification do 10 Bali cows in Panca Tunggal Village, South Bangka Regency and 11 Bali cattle in Temberan Village, Pangkalpinang City. The bali cattle serum used for virus identification was centrifuged to obtain buffycoat. The PCR-RT technique is carried out in several stages, namely sampling, buffycoat making, extraction, master mix making, amplification, agar manufacture, electrophoresis and visualization. Based on the test, it was found that A1 and A2 bali cattle from Panca Tunggal Village, South Bangka Regency indicated the presence of the Jembrana virus. So that this Polymerase Chain Reaction Technique can help detect the presence of the Jembrana virus in bali cattles.
 
Publisher Research Center for Biology-Indonesian Institute of Sciences
 
Contributor Jurusan Biologi Fakultas Pertanian Perikanan dan Biologi Universitas Bangka Belitung, Kepala Dinas Pertanian Provinsi Bangka Belitung Bapak Juaidi, SP. MP., Kepala Laboratorium Kesehatan Hewan dan Kesehatan Masyarakat Veteriner dan Staf Laboratorium
 
Date 2021-12-27
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/4055
10.14203/beritabiologi.v21i3.4055
 
Source BERITA BIOLOGI; Vol 21, No 3 (2021): Berita Biologi; 345-348
BERITA BIOLOGI; Vol 21, No 3 (2021): Berita Biologi; 345-348
2337-8751
0126-1754
 
Language eng
 
Relation http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/4055/pdf_1
 
Rights Copyright (c) 2021 BERITA BIOLOGI
 

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