Physicochemical and microbial characterization of an oil pit from Zulia state, Venezuela

Ciencia

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Title Physicochemical and microbial characterization of an oil pit from Zulia state, Venezuela
Caracterización fisicoquímica y microbiológica de una fosa petrolera del estado Zulia, Venezuela
 
Creator Díaz-Borrego, Laugeny
Briceño, Beltrán
Mora, Roberta
Vera, Alexandra
Rosales, Nestor
González, Lenin
Marín, Julio
Aiello-Mazzarri, Cateryna
Morales, Ever
 
Subject Physicochemical characteristics
bacteria
fungi
microalgae
rotifers
oil pit
Características fisicoquímicas
bacterias
hongos
microalgas
rotíferos
fosa petrolera
 
Description Oil pits are poorly designed soil excavations used to store hazardous wastes, which can leak into the environment causing serious damage to the ecosystem. They are considered a hostile environment for life due to the toxic compounds they possess, but there are microbial communities that adapt to this environment and can be used for their recovery. In this work, samples of water, sediment and oil from an oil well in the state of Zulia, Venezuela, were characterized physicochemically and microbiologically using standardized laboratory techniques. The water presented the following physicochemical characteristics: temperature: (30 ± 2)ºC, pH: 5.10-5.90, total alkalinity: 8.00 mg CaCO3 / L, Salinity: 0 UPS, BOD5.20: <0.10 mg/L, COD: 184.6-215.4 mg/L, dissolved oxygen: 8.00-8.97 mg/L, SST: 61-235 mg/L, SSV: 35-47 mg/L, nitrites: <0.0020, nitrates: <0.025 mg/L, NTK: 2.78-5.57 mg/L, orthophosphates: <0.15 mg/L, total phosphorus: 0.47-0.74 mg/L, HTP: 673.4- 821.7 mg/L, saturated: 86.73%, aromatics, resins and asphaltenes: <4.5%, Ni and V: <0.02 mg/L. The sediment presented organic matter: (4.09 ± 0.95)%, NTK: 728.33 ± 45.25 mg/Kg, total phosphorus: 40.37 ± 11.80 mg/Kg, crude in sediment: 13.05 ± 0.75%, saturated: 48.5%, aromatic: 33.5%, resins: 6.5% and asphaltenes: 11.5%. In crude oil, the following were determined: saturated: 48.1%, aromatic: 23.6%, resins: 19.4%, and asphaltenes: 8.9%. Microbial populations were quantified obtaining: (4.15 ± 1.43) x 106 org/mL of photosynthetic microorganisms (microalgae and cyanobacteria), (2.71 ± 0.49) x 107 CFU/mL of fungi in petrolized water and (2.25 ± 0.24) x 106 CFU/g in sediment, (2.09 ± 1.50) x 101 org/mL of rotifers in water and a bacterial population of (2.45 ± 0.34) x 105 CFU/mL in petrolized water and (2.83 ± 0.26) x 105 CFU/g in sediment. It is concluded that despite the fact that physicochemical conditions such as acidic pH in the water, low concentration of nutrients and organic matter, and high concentration of hydrocarbons were present in the oil well, microbial populations adapted to these conditions were found that could be used as biocatalysts for the bioremediation of the oil well.
Las fosas petroleras son excavaciones de terreno con diseños deficientes que se utilizan para almacenar desechos peligrosos, los cuales se pueden filtrar al ambiente causando graves daños al ecosistema. Son consideradas un ambiente hostil para la vida por los compuestos tóxicos que poseen, aunque existen comunidades microbianas que se adaptan a este ambiente y pueden ser utilizadas para su recuperación. En este trabajo se caracterizaron fisicoquímica y microbiológicamente muestras de agua, sedimento y petróleo de una fosa petrolera del estado Zulia, Venezuela, mediante técnicas estandarizadas de laboratorio. El agua presentó las siguientes características fisicoquímicas: temperatura: (30±2)ºC, pH: 5,10-5,90, alcalinidad total:8,00 mg CaCO3/L, Salinidad: 0 UPS, DBO5,20: <0,10 mg/L, DQO: 184,6-215,4 mg/L, oxígeno disuelto: 8,00-8,97 mg/L, SST: 61-235, SSV: 35-47 mg/L, nitritos: <0.0020, nitratos: <0,025 mg/L, NTK: 2,78-5,57 mg/L, ortofosfatos: <0,15 mg/L, fósforo total: 0,47-0,74 mg/L, TPH: 673,4-821,7 mg/L, saturados: 86,73%, aromáticos, resinas y asfaltenos: <4,5%, Ni y V: <0,02 mg/L. El sedimento presentó materia orgánica: (4,09 ± 0,95)%, NTK: (728,33 ± 45,25) mg/Kg, fósforo total: (40,37 ± 11,80) mg/Kg, crudo en sedimento: (13,05 ± 0,75)%, saturados: 48,5%, aromáticos: 33,5%, resinas: 6,5% y asfaltenos: 11,5%. En el crudo se determinaron: saturados: 48,1%, aromáticos: 23,6%, resinas: 19,4% y asfaltenos: 8,9%. Se cuantificaron las poblaciones microbianas obteniendo: (4,15 ± 1,43) x 106 org/mL de microorganismos fotosintéticos (microalgas y cianobacterias), (2,71 ± 0,49) x 107 UFC/mL de hongos en agua petrolizada y de (2,25 ± 0,24) x 106 UFC/g en sedimento, (2,09 ± 1,50) x 101 org/mL de rotíferos en el agua y una población bacteriana de (2,45 ± 0,34) x 105 UFC/ml en agua petrolizada y de (2,83 ± 0,26) x 105 UFC/g en sedimento. Se concluye que a pesar de que en la fosa petrolera se presentaron condiciones fisicoquímicas como pH ácido en el agua, baja concentración de nutrientes y de materia orgánica, y alta concentración de hidrocarburos, se encontraron poblaciones microbianas adaptadas a estas condiciones que podrían emplearse como biocatalizadores para la biorremediación de la fosa petrolera.
 
Publisher Facultad Experimental de Ciencias de la Universidad del Zulia
 
Date 2019-07-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
text/html
 
Identifier http://produccioncientificaluz.org/index.php/ciencia/article/view/37126
10.5281/zenodo.5592942
 
Source Ciencia; Vol. 27 Núm. 1-2 (2019): Enero-Junio; 5-13
1315-2076
10.5281/zenodo.5592803
 
Language spa
 
Relation http://produccioncientificaluz.org/index.php/ciencia/article/view/37126/40364
http://produccioncientificaluz.org/index.php/ciencia/article/view/37126/40365
 

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