Paper Chromatographic Separation of Plant Pigments a New Approach by using Akshya-Swagatika Solvent and Mobile Chromatogram Detection System (MCDS)

International Journal for Research in Applied Sciences and Biotechnology

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Title Paper Chromatographic Separation of Plant Pigments a New Approach by using Akshya-Swagatika Solvent and Mobile Chromatogram Detection System (MCDS)
 
Creator Akshya Kumar Mishra
Swagatika Padhan
Arpita Behera
Madhusmita Naik
Jharana Nag
Rukmani Sahu
Hemant Raut
Gulshan Dandsena
Saudamini Karual
Rinki Harijan
Poonam Pradhan
R. Gowri Kalyani
Hira Bariha
Prangya Parimita Sahu
Balkishan Sahu
Sangita Jagat
Digesh Kumar Dandsena
Pranjalee Sharma
Duryodhan Pandey
Jagnyaseni Sahu
Ankita Sahu
Rajesh Ku. Sahu
Bhumika Sahu
Narendra Raut
Neetu Pradhan
Neha Sahu
Karuna Kar Pradhan
Bhomishma Chandrakar
Girija Sahu
 
Subject Plant pigments
Paper Chromatography
Akshya-Swagatika solvent
Mobile chromatogram detection system (MCDS)
 
Description Chromatography is a term that refers to a group of laboratory techniques for separating mixtures. Chromatography works on the premise of solute partitioning between two phases or solvents. The technique of paper chromatography is commonly used to separate plant pigments based on their molecular weight. Plant pigments include chlorophyll-b, chlorophyll-a, carotenoid, and xanthophyll, which all have various molecular weights, colours, and absorption maxima. In this study, an attempt was made to see how a new solvent (Akshya-Swagatika solvent) can be used to separate plant pigments using paper chromatography, as well as a new detection method developed by us known as Mobile chromatogram detection system (MCDS) that can be used for compound identification and photographing. As shown in the table and photograph, pigments are separated by molecular weight and band colour, with carotenoid having the highest RF value and chlorophyll-b having the lowest. It was obvious that the Akshya-Swagatika solution could be used to separate plant pigments in paper chromatography. Photograph taken with a mobile chromatogram detection system that is much clearer than normal one. In developing countries, both solvent and detection systems are useful in explaining paper chromatography in a cost-effective manner. The MCDS detection system is cost-effective fast method which replaces traditional sophisticated detection procedures.
 
Publisher Vandana Publications
 
Date 2021-11-02
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://www.ijrasb.com/ojs/index.php/ojs-ijrasb/article/view/456
10.31033/ijrasb.8.6.1
 
Source International Journal for Research in Applied Sciences and Biotechnology; Vol. 8 No. 6 (2021): November Issue; 1-3
2349-8889
 
Language eng
 
Relation https://www.ijrasb.com/ojs/index.php/ojs-ijrasb/article/view/456/343
 
Rights Copyright (c) 2021 International Journal for Research in Applied Sciences and Biotechnology
https://creativecommons.org/licenses/by-nc-nd/4.0
 

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