Extraction of Lipopeptide from Bacillus subtilis and Histological Study on Albino Rats Tissue

International Journal for Research in Applied Sciences and Biotechnology

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Field Value
 
Title Extraction of Lipopeptide from Bacillus subtilis and Histological Study on Albino Rats Tissue
 
Creator Ghofran Filaih Abd AL-Hussan
Waleed Jaleel Abed AL-Kelaby
Ali Yas Khudhair AL-Ameri
 
Subject Bacillus subtilis
Lipopeptide
Albino rats
tissues
 
Description Introduction and Aim: An extract of the lipopeptides from Bacillus subtilis has been found to be extremely useful in antimicrobial applications. Specifically, the goal of this study is to manufacture lipopeptide and assess the safety of this substance on the tissues and organs of laboratory rats.
Materials and Methods: Bacillus subtilis bacteria were isolated and identified at the postgraduate microbiology laboratory in the department of biology at Kufa University's College of Science. The bacteria became active after 24 hours of growth in the brain broth infusion broth medium. In order to determine the bacteria's capacity to produce lipopeptide. On nutrient agar medium, a quantity of lipopeptide was synthesized utilizing HCL for sediment, yielding an estimated amount of 1.5 gm during a two-month period, then the product was partially purified and lyophilized using a lyophilizer. For one week, the lipopeptide was administered on albino rat tissues such as the kidney, liver, spleen, and small intestine to determine its safety.
Results: The results showed no damage or any changes on tested tissues compared with control treatment and all of the previously mentioned organ tissues were completely intact and this is evidence of the safety of the lipopeptide extract for use.
Conclusions: It was discovered that lipopeptide had no effect on the organs that were utilized in the experiment and that it is safe for human consumption.
 
Publisher Vandana Publications
 
Date 2021-10-19
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://www.ijrasb.com/ojs/index.php/ojs-ijrasb/article/view/452
10.31033/ijrasb.8.5.27
 
Source International Journal for Research in Applied Sciences and Biotechnology; Vol. 8 No. 5 (2021): September Issue; 183-186
2349-8889
 
Language eng
 
Relation https://www.ijrasb.com/ojs/index.php/ojs-ijrasb/article/view/452/339
 
Rights Copyright (c) 2021 International Journal for Research in Applied Sciences and Biotechnology
https://creativecommons.org/licenses/by-nc-nd/4.0
 

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