Comparative study of the phases of hemostasis in two lines of laboratory rats

Revista GICOS

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Title Comparative study of the phases of hemostasis in two lines of laboratory rats
Estudio comparativo de las fases de la hemostasia en dos líneas de ratas de laboratorio Comparative study of the phases of hemostasis in two lines of laboratory rats
 
Creator Navas, Lilibeth
Villasana, Katiusca
León, Wanda
Montero, Yepsys
Quiñonez, Belkis
 
Subject
Keywords: hemostasis, blood coagulation, hemostasis test, Wistar rats, Sprague-Dawley rats.

hemostasia; coagulación sanguínea; pruebas de hemostasia; ratas Wistar; ratas Sprague-Dawley.
 
Description The objective of the research was to compare the hemostasis phases in laboratory rats of the BIOU lines: Wistar and BIOU: Sprague-Dawley. Methodology: 10 rats from each of the lines were used, produced by the Bioterio of the University of Los Andes, with body weight between 370 and 400 g, it was determined: bleeding time, platelet count, prothrombin time (PT), partial time of activated thromboplastin (aPTT), clotting time and fibrinogen. Results: bleeding time and PT were significantly lower in BIOU: Sprague-Dawley rats (p = 0.002 and p = 0.011 respectively). There was no significant difference in platelet count, aPTT, clotting time and fibrinogen between both lines of rats (p> 0.05). Conclusion: differences were observed between the BIOU: Sprague-Dawley and BIOU: Wistar laboratory rats, both in the primary hemostasis as in secondary hemostasis; specifically, bleeding time and PT were significantly lower in BIOU: Sprague-Dawley rats. The differences found could be explained by variations in the genetic bases of each line of rats, which influence platelet function or the expression and / or activity of coagulation factors, anticoagulant proteins and components of the fibrinolytic system.
El objetivo de la investigación fue comparar las fases de la hemostasia, en ratas de laboratorio de las líneas BIOU: Wistar y BIOU: Sprague-Dawley. Metodología: se utilizaron 10 ratas de cada una de las líneas, producidas por el Bioterio de la Universidad de Los Andes, con peso corporal comprendido entre 370 y 400 g, se determinó: tiempo de sangría, contaje plaquetario, tiempo de protrombina (TP), tiempo parcial de tromboplastina activado (TPTa), tiempo de coagulación y fibrinógeno. Resultados: el tiempo de sangría y el TP fueron significativamente menores en las ratas BIOU:Sprague-Dawley (p=0,002 y p=0,011 respectivamente). No hubo diferencia significativa en el contaje plaquetario, TPTa, tiempo de coagulación y fibrinógeno entre ambas líneas de ratas (p>0,05) Conclusión: se evidenciaron diferencias entre las ratas de laboratorio BIOU: Sprague-Dawley y BIOU: Wistar, tanto en la hemostasia primaria como en la hemostasia secundaria; específicamente el tiempo de sangría y el TP fueron significativamente menores en las ratas BIOU: Sprague-Dawley. Las diferencias halladas podrían explicarse por variaciones en las bases genéticas de cada línea de ratas, que influyen en la función plaquetaria o en la expresión y/o actividad de los factores de coagulación, proteínas anticoagulantes y componentes del sistema fibrinolítico.  The objective of the research was to compare the hemostasis phases in laboratory rats of the BIOU lines: Wistar and BIOU: Sprague-Dawley. Methodology: 10 rats from each of the lines were used, produced by the Bioterio of the University of Los Andes, with body weight between 370 and 400 g, it was determined: bleeding time, platelet count, prothrombin time (PT), partial time of activated thromboplastin (aPTT), clotting time and fibrinogen. Results: bleeding time and PT were significantly lower in BIOU: Sprague-Dawley rats (p = 0.002 and p = 0.011 respectively). There was no significant difference in platelet count, aPTT, clotting time and fibrinogen between both lines of rats (p> 0.05). Conclusion: differences were observed between the BIOU: Sprague-Dawley and BIOU: Wistar laboratory rats, both in the primary hemostasis as in secondary hemostasis; specifically, bleeding time and PT were significantly lower in BIOU: Sprague-Dawley rats. The differences found could be explained by variations in the genetic bases of each line of rats, which influence platelet function or the expression and / or activity of coagulation factors, anticoagulant proteins and components of the fibrinolytic system.
 
Publisher GICOS
 
Contributor

 
Date 2021-10-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion



 
Format application/pdf
 
Identifier http://erevistas.saber.ula.ve/index.php/gicos/article/view/17418
 
Source GICOS; Vol. 6, Núm. 4 (2021): julio-diciembre; 71-84
2610-797X
 
Language spa
 
Relation http://erevistas.saber.ula.ve/index.php/gicos/article/view/17418/21921928616
 
Rights Copyright (c) 2021 GICOS
 

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