Screening for the Production and Characterization of L-asparaginase from Endophytic Fungi

International Journal for Research in Applied Sciences and Biotechnology

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Field Value
 
Title Screening for the Production and Characterization of L-asparaginase from Endophytic Fungi
 
Creator Harjeet Singh
Shweta Sao
 
Subject L-asparaginase
fungal endophytes
acute lymphoblastic leukemia
 
Description L-asparaginase (EC 3.5.11. L-asparagine amidohydrolase) is first enzyme, studied very intensively in human beings with regard to its anti-tumor potential against tumor of lymphoid precursor, acute lymphoblastic leukemia (ALL). The current drugs are suffering from many side effects like immune suppression, infertility, secondary neoplasm. The immunogenic complications associated with its present microbial sources Escherichia coli; Erwinia carotovora limits its medicinal frontier. So there exists a need of switching to novel natural sources to serve as non-immunogenic and better production sources of L-asparaginase. In the present study, four cultures of fungal endophytes viz. TSF-1, TSF-2, TSF-3 and TSF-4 selected on the basis of primary and secondary screening was carried on with L-asparagine as a sole carbon and nitrogen source and phenol red as pH indicator. The maximum protein content was observed to be present in TSF-2 i.e. 2.727 mg /mL and possessed maximum activity of 6.054 Units/ml. Sample was separated by SDS-PAGE, stained by silver staining, showed a single band with molecular weight of approximately ~45kDa.
 
Publisher Vandana Publications
 
Date 2021-10-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://www.ijrasb.com/ojs/index.php/ojs-ijrasb/article/view/450
10.31033/ijrasb.8.5.24
 
Source International Journal for Research in Applied Sciences and Biotechnology; Vol. 8 No. 5 (2021): September Issue; 168-173
2349-8889
 
Language eng
 
Relation https://www.ijrasb.com/ojs/index.php/ojs-ijrasb/article/view/450/336
 
Rights Copyright (c) 2021 International Journal for Research in Applied Sciences and Biotechnology
https://creativecommons.org/licenses/by-nc-nd/4.0
 

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