Activity Test, Selectivity, Stability of Chitinase on Amobil Chitosan Membranes

International Journal of Multicultural and Multireligious Understanding

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Field Value
 
Title Activity Test, Selectivity, Stability of Chitinase on Amobil Chitosan Membranes
 
Creator Hamsina, Hamsina
Tang, M
Hasani, Erni Indrawati Ruslan
 
Subject
Chitinase Enzyme; Enzyme Immobilization; Chitosan Membrane
 
Description The use of enzymes for industrial functions needs enzymes that are stable, selective and might be used repeatedly. The aim of the study was to determine the chitinase enzyme's function, selectivity, and stability in amobil chitosan membranes. The research method consisted of stages: production of the chitinase enzyme which included the manufacture of chitin colloidal substrate, rejuvenation of thermophilic bacteria, preparation of the inoculum and determining the optimum time of production, fractionation of ammonium sulfate, chitinase enzyme immobilization technique and activity, stability and selectivity test of amobil enzyme. The results demonstrated that chitinase activity, which incorporates the optimum temperature and thus the optimum concentration of production within the immobilization technique, had an optimum temperature of 65oC on day 4 of production time with an OD value of 0.9876. The selectivity of amobil chitinase with metal ions Cd (II), Pb (II), Zn (II), and Hg (II) demonstrated that amobil chitinase was selective for these ions. Eamobil chitinase was heat stable at 55-75oC and resistant to organic solvents, suggesting that it could be used repeatedly.
 
Publisher University of Hamburg
 
Contributor
 
Date 2021-08-18
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://ijmmu.com/index.php/ijmmu/article/view/2750
10.18415/ijmmu.v8i7.2750
 
Source International Journal of Multicultural and Multireligious Understanding; Vol 8, No 7 (2021): July 2021; 453-464
2364-5369
2364-5369
10.18415/ijmmu.v8i7
 
Language eng
 
Relation http://ijmmu.com/index.php/ijmmu/article/view/2750/2418
 
Rights Copyright (c) 2021 International Journal of Multicultural and Multireligious Understanding
http://creativecommons.org/licenses/by-nc-nd/4.0
 

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