Study of Phenytoin Effect on the genes involved in glucose and lipid metabolism expression in liver: A mouse model study

Journal of Current Biomedical Reports

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Title Study of Phenytoin Effect on the genes involved in glucose and lipid metabolism expression in liver: A mouse model study
 
Creator Samimi, Fatemeh
Ahadi, Ali Mohammad
Boroomand, Nafiseh
Mohammadzadeh Rostami, Farzaneh
 
Subject Genetic; Biochemistry
Phenytoin; Akt1; Leptin; Adipoq; GLUT4
 
Description Phenytoin as an anti-seizure medication, is useful for the prevention of tonic-clonic seizures and focal seizures. In this study we focused on the probable effects of Phenytoin drug on gene expression profile of liver related to lipid metabolism balance in mouse as a model. In this study, a group including 7 male mice of BALB/c were treated with phenytoin 3–5 mg/kg/day orally and a group including 7 male mice of BALB/c were took standard food. Liver tissue samples were isolated. Total RNA was extracted and cDNA was synthesized. Expression of Akt1, Leptin, Adipoq and GLUT4 genes was measured using Real-time RT-PCR method. Results showed an increase about 15 and 3 fold changes in Akt1 and Adipoq gene expression respectively in treatment group compare to control mice. Also, we detected decreasing in Leptin and GLUT4 genes expression in the mice treated with phenytoin drug. Several studies indicated that phenytoin can promote hyperglycemia in human and animal. We proposed here that this effects may resulted from an interference between the phenytoin drug and gene expression profile in liver. Decreasing of leptin level here may be a result of glucose level elevation in blood that can induce a satiety situation result in decrease of leptin production. It may that Akt1 gene expression is increased to compensate the low level of GLUT4 protein. We concluded that phenytoin is a relatively high-risk antiepileptic drug for obesity and metabolic syndrome, but more studies are needed.
 
Publisher ParsPublisher
 
Contributor
 
Date 2021-06-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
 
Identifier https://jcbior.com/index.php/JCBioR/article/view/84
10.52547/JCBioR.2.2.62
 
Source Journal of Current Biomedical Reports; Vol 2, No 2 (2021). April-June; 62-68
2717-1906
 
Language eng
 
Relation https://jcbior.com/index.php/JCBioR/article/view/84/PDF
https://jcbior.com/index.php/JCBioR/article/view/84/Docx
https://jcbior.com/index.php/JCBioR/article/view/84/Fa
https://jcbior.com/index.php/JCBioR/article/downloadSuppFile/84/63
 
Rights Copyright (c) 2021 © The Author(s)
https://creativecommons.org/licenses/by-nc/4.0
 

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