Detecting of biofilm formation in the clinical isolates of Pseudomonas aeruginosa and Escherichia coli: an evaluation of different screening methods

Journal of Current Biomedical Reports

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Field Value
 
Title Detecting of biofilm formation in the clinical isolates of Pseudomonas aeruginosa and Escherichia coli: an evaluation of different screening methods
 
Creator Mohsenzadeh, Amin
Fazel, Alireza
Bavari, Shirin
Borji, Sanaz
Pourasghar, Sahar
Azimi, Taher
Sabati, Hoda
 
Subject Microbiology
Biofilm detection; Microtiter plate assay; Congo red agar; Tube method
 
Description Biofilm producer bacteria cause nosocomial, chronic, and recurrent human infections. It is hard to treat biofilm-embedded bacteria because they are more resistant to antimicrobials than planktonic bacteria. The present study aimed to investigate different methods for detecting biofilms in the clinical isolates of Escherichia coli and Pseudomonas aeruginosa. The study was carried out at the Department of Microbiology, Ardabil Branch, Islamic Azad University, Ardabil, Iran, from January 2019 to June 2019. A total of 320 clinical samples were collected from educational and medical centers in Tehran, Iran which from them 100 isolates of E. coli and P. aeruginosa were identified by standard microbiological procedures and subjected to biofilm detection methods. Biofilm detection was tested by Congo red agar (CRA), tube method (TM), microtiter plate assay (MTPA). The MTPA was considered to be superior to CRA and tube TM. From the total of 100 clinical isolates, MTPA detected 38 (38%) isolates as biofilm-positive phenotype, of which 30 as strong, and 8 as moderate biofilm-forming isolates. It can be concluded from the current study that the MTPA is a more quantitative and dependable assay for the detection of biofilm-forming microorganisms as compared to other methods, and it can be recommended as a general screening method for the detection of biofilm-producing bacteria in laboratories.
 
Publisher ParsPublisher
 
Contributor
 
Date 2021-06-03
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
application/pdf
 
Identifier https://jcbior.com/index.php/JCBioR/article/view/78
10.52547/JCBioR.2.2.56
 
Source Journal of Current Biomedical Reports; Vol 2, No 2 (2021). April-June; 56-61
2717-1906
 
Language eng
 
Relation https://jcbior.com/index.php/JCBioR/article/view/78/PDF
https://jcbior.com/index.php/JCBioR/article/view/78/Fa
 
Rights Copyright (c) 2021 © The Author(s)
https://creativecommons.org/licenses/by-nc/4.0
 

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