Isolation and Screening of Rhizosphere and Endophytic Fungus from Moringa as Germination Inducing Agents on Rice Seed

AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian

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Title Isolation and Screening of Rhizosphere and Endophytic Fungus from Moringa as Germination Inducing Agents on Rice Seed
Isolasi dan Seleksi Cendawan Rhizosfer dan Endofit asal Tanaman Kelor sebagai Agens Penginduksi Perkecambahan pada Benih Padi
 
Creator Mirsam, Hishar
Masluki, Masluki
Mutmainnah, Mutmainnah
 
Description Rhizosphere and endophytic fungi are functional types of microbes capable of producing secondary metabolites that can affect plant growth directly or indirectly. This study aims to isolate and test the rhizosphere and endophytic fungi' ability from Moringa (Moringa oleifera L.) against rice seeds' viability and vigor. Fungus exploration was carried out on soil samples in the rhizosphere and stem and leaf tissue of healthy Moringa plants. Isolation of fungi from the rhizosphere was carried out using 10-2 and 10-3 dilution techniques, while the isolation of endophytic fungi was carried out on the leaf and stem tissue of Moringa, then cultured on potato dextrose agar (PDA) medium. The pathogenicity test of fungi and its effect on in-vitro rice seed germination using the blotter test method, namely growing 25 rice seeds on seven-day old fungal isolates. Nineteen fungal isolates were isolated and collected from the parts of the Moringa plant. Pathogenicity observations showed that there were five fungal isolates as potential pathogens, namely isolates RF2, RF5, RF6, RF8, and EDF6. A total of four fungal isolates tested consistently showed a positive effect on seed viability and vigor with a value of ≥90%, namely isolates RF4, EDF1, EDF2, and EDFbt3.
Cendawan rhizosfer dan endofit merupakan jenis mikroba fungsional yang mampu memproduksi metabolit sekunder yang dapat memengaruhi pertumbuhan tanaman baik secara langsung atau tidak langsung. Penelitian ini bertujuan mengisolasi dan menguji kemampuan cendawan rhizosfer dan endofit asal tanaman kelor terhadap viabilitas dan vigor kecambah benih padi. Eksplorasi cendawan dilakukan terhadap sampel tanah di bagian rhizosfer serta jaringan batang dan daun tanaman kelor sehat. Isolasi cendawan dari tanah bagian rhizosfer dilakukan dengan teknik pengenceran 10-2 dan 10-3, sedangkan cendawan endofit dilakukan pada jaringan daun dan batang tanamn kelor, kemudian dibiakkan pada media medium potato dextrose agar (PDA). Uji patogenisitas cendawan dan pengaruhnya terhadap perkecambahan benih padi secara in-vitro dengan metode blotter test, yaitu dengan cara menumbuhkan benih padi sebanyak 25 butir pada isolat cendawan berumur 7 hari. Sebanyak sembilan belas isolat cendawan berhasil diisolasi dan dikoleksi dari berbagai bagian tanaman kelor. Pengamatan patogenisitas menunjukkan terdapat lima isolat cendawan yang berpotensi sebagai patogen, yaitu isolat RF2, RF5, RF6, RF8, dan EDF6. Sebanyak empat isolat cendawan yang telah diuji konsisten memberikan pengaruh positif terhadap viabilitas dan vigor benih benih dengan nilai ≥90% yaitu isolate RF4, EDF1, EDF2, dan EDFbt3
 
Publisher Universitas Bangka Belitung
 
Date 2021-05-28
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://agrosainstek.ubb.ac.id/index.php/agrosainstek/article/view/227
10.33019/agrosainstek.v5i1.227
 
Source AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian; Vol 5 No 1 (2021): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian; 34-43
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian; Vol 5 No 1 (2021): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian; 34-43
2579-843X
2615-2207
10.33019/agrosainstek.v5i1
 
Language eng
 
Relation https://agrosainstek.ubb.ac.id/index.php/agrosainstek/article/view/227/63
 
Rights Copyright (c) 2021 Hishar Mirsam, Masluki Masluki, Mutmainnah Mutmainnah
http://creativecommons.org/licenses/by/4.0
 

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