Isolasi dan Karakterisasi Cendawan Pathogen Daun Jagung Manis (Zea mays) Varietas Talenta menggunakan Metode Direct Plating Dan Moist Chamber di BBPP Ketindan, Jawa Timur

BioEksakta : Jurnal Ilmiah Biologi Unsoed

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Title Isolasi dan Karakterisasi Cendawan Pathogen Daun Jagung Manis (Zea mays) Varietas Talenta menggunakan Metode Direct Plating Dan Moist Chamber di BBPP Ketindan, Jawa Timur

 
Creator fadilah, nurul
Rahayu, Yuni Sri
Andriani, Lutfi Tri
 
Description Market demanding for corn increases with the years. The greatest request of all types of maize is the sweet corn (Zea mays) talenta variety. However, domestic productions arenot sufficient for this corn. This is due to problems such as corn disease which drastically derailed yields. This disease comes from the category of pathogenic fungi. This study aims to isolate and characterize fungal pathogens in sweet corn leaves (Zea mays) of talenta varieties taken from agricultural land, Ketindan Agricultural Training Center, East Java. This research is an experimental study using  Completely Randomized  Design (CRD). The insulation method used direct plating and moist chamber. Characterization and identification based on macroscopic characters include: old colony shape, old colony color, old colony texture, young colony shape, young colony color, and young colony texture. Microscopic characters include hyphal wall, type of hyphae branching, presence or absence of septa, presence or absence of clamp connections, sporangium shape, sporangium color, spore shape, spore color. The results of the direct plating method obtained 5 species of pathogenic fungi, including: Helminthosporium sp., Rhizoctonia sp., Peronosclerospora spp., Puccinia sp., And Trichometaspheria sp., While the moist chamber method obtained 2 species of pathogenic fungi, namely: Helminthosporium sp. and Rhizoctonia sp.
Permintaan pasar akan jagung meningkat dengan seiring berjalannya tahun. Permintaan terbesar dari semua jenis jagung adalah jagung manis (Zea mays) varietas talenta. Namun, produksi dalam negeri tidak mencukupi permintaan jagung tersebut. Hal ini dikarenakan adanya masalah seperti, penyakit jagung yang menggagalkan hasil panen secara drastis. Penyakit ini berasal dari kategori cendawan pathogen. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi cendawan pathogen pada daun jagung manis (Zea mays) varietas talenta yang diambil dari Lahan Pertanian, Balai Besar Pelatihan Pertanian (BBPP) Ketindan, Jawa Timur. Penelitian ini merupakan penelitian eksperimental menggunakan Rancangan Acak lengkap (RAL). Metode isolasi yang digunakan adalah direct plating dan moist chamber. Karakterisasi dan Identifikasi berdasarkan karakter makroskopis meliputi: bentuk koloni tua, warna koloni tua, tekstur koloni tua, bentuk koloni muda, warna koloni muda, dan tekstur koloni muda. Karakter mikroskopis meliputi dinding hifa, tipe percabangan hifa, ada tidaknya septa, ada tidaknya sambungan apit (clamp connection), bentuk sporangium, warna sporangium, bentuk spora, warna spora). Hasil dari metode direct plating didapatkan 5 spesies jamur pathogen antara lain: Helminthosporium sp., Rhizoctonia sp., Peronosclerospora spp., Puccinia sp., dan Trichometaspheria sp., sedangkan metode moist chamber didapatkan 2 spesies jamur pathogen yaitu: Helminthosporium sp. dan Rhizoctonia sp.
 
Publisher Fakultas Biologi Universitas Jenderal Soedirman
 
Date 2021-04-10
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://jos.unsoed.ac.id/index.php/bioe/article/view/3777
10.20884/1.bioe.2020.2.3.3777
 
Source BioEksakta : Jurnal Ilmiah Biologi Unsoed; Vol 3 No 1 (2021): Bioeksakta; 20-25
2714-8564
 
Language eng
 
Relation http://jos.unsoed.ac.id/index.php/bioe/article/view/3777/2349
 
Rights Copyright (c) 2021 BioEksakta : Jurnal Ilmiah Biologi Unsoed
 

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