The use of cinnamon oil as antibacterial agent to eliminate some antibiotic-resistant bacteria isolated from water sources

Acta Fytotechnica et Zootechnica

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Title The use of cinnamon oil as antibacterial agent to eliminate some antibiotic-resistant bacteria isolated from water sources
 
Creator Al Yousef, Sulaiman Ali
 
Description Article Details: Received: 2020-07-09 | Accepted: 2020-10-14 | Available online: 2021-03-31 https://doi.org/10.15414/afz.2021.24.01.1-8Extended-spectrum β-lactamases (ESBL) are enzymes produced by Gram-negative microorganisms, which may be resistant to commonly used antibiotics. The purpose of this research was to estimate the bactericidal effects of cinnamon oil on ESBLproducing bacteria. In this study, 227 water samples were collected from wells in Hafr Al-Batin, Saudi Arabia. The samples were cultured on a cystine lactose electrolyte-deficient (CLED) medium. A MicroScan system was used to identify bacteria and also for antimicrobial susceptibility test. Activity of crud cinnamon oil and its fractions were detected by determining the minimum inhibitory concentration (MIC) against the ESBL-producing bacteria. Morphological changes of the treated bacteria were observed and oil compounds was investigated. The culture was positive on the CLED medium in 170 out of 227 water samples. In 170 CLED-positive isolates, E. coli was the most common organism, followed by K. pneumoniae. The results showed that 100% of K. pneumoniae isolates were completely resistant to ampicillin (100%), then by mezlocillin (92.5%), cefazolin, and cefuroxime (77.5%). Also, 86.9% of E. coli isolates were the most resistant to ampicillin, followed by mezlocillin (83%). 82% of K. pneumoniae and 89% of E. coli isolates were confirmed by phenotypic confirmatory disc diffusion test (PCDDT) as ESBL-producers. The cinnamon oil activity was only concentrated in the oxygenated fraction. The MICs of the oxygenated fraction were 80 and 20 µl/mL at 105 CFU of ESBL-producing E. coli and K. pneumoniae, respectively. 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Publisher Acta Fytotechnica et Zootechnica
 
Contributor Sabry Mahmoud, King Khaled Hospital, University of Hafr Al Batin
 
Date 2021-04-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://www.acta.fapz.uniag.sk/journal/index.php/on_line/article/view/710
 
Source Acta Fytotechnica et Zootechnica; Vol 24, No 1 (2021)
1336-9245
1336-9245
 
Language eng
 
Relation http://www.acta.fapz.uniag.sk/journal/index.php/on_line/article/view/710/pdf
 
Rights Copyright (c) 2021 Acta Fytotechnica et Zootechnica
 

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