Lymphocyte Proliferation and Nitric Oxide-Producing Activities of Lupeol Isolated From Red Dragon Fruit (Hylocereus polyrhizus) Extract

Molecular and Cellular Biomedical Sciences

View Publication Info
 
 
Field Value
 
Title Lymphocyte Proliferation and Nitric Oxide-Producing Activities of Lupeol Isolated From Red Dragon Fruit (Hylocereus polyrhizus) Extract
 
Creator Wahdaningsih, Sri
Wahyuono, Subagus
Riyanto, Sugeng
Murwanti, Retno
 
Description Background: Hylocereus polyrhizus has activities as antimicrobial agent, anti-hypercholesterolemia, anti-diabetic (diabetes mellitus), cardiovascular risk reduction, health supplement, and melanoma cell suppression. The extracts from the peels of H. polyrhizus were able to increase phagocytic ability, cell numbers and leukocytes and to influence relative spleen weights in the formation of body immune system in male rats. The fruit peels contained phenolics, flavonoids, carotenoids, and anthocyanins. This study investigated the active compounds of H. polyrhizus peels, which are able to increase immune system of human body.Materials and method: In vitro assay was applied to examine the active compounds, identified as lupeol, obtained from isolated extract of red dragon fruit for their lymphocyte proliferation and nitric oxide (NO)-producing activities. Lymphocyte proliferation assay was performed with 3-4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) method. The cell control was lymphocyte cell suspension in RPMI medium added with phytohaemaglutinine (PHA). The NO measurement was conducted with nitric solvent and Greiss reagent.Results: The ANOVA analysis of the average optical density (OD) of lymphocyte proliferation showed that the addition of isolated lupeol at the concentrations of 6.25, 12.5, 25, 50 and 100 µg/mL were able to improve lymphocyte proliferation and activate the NO production in the rats with treatment of positive control. Conclusion: Isolated lupeol at concentrations of 6.25, 12.5, 25, 50 and 100 µg/mL revealed significant difference with medium control and cell control. It was able to increase effects on lymphocyte proliferation and NO production. Therefore, the lupeol which was isolated might have high potential to be an immunostimulant.Keywords: Hylocereus polyrhizus, lupeol, lymphocyte proliferation, nitric oxide production
 
Publisher Cell and BioPharmaceutical Institute
 
Contributor
 
Date 2021-03-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier https://cellbiopharm.com/ojs/index.php/MCBS/article/view/166
10.21705/mcbs.v5i1.166
 
Source Molecular and Cellular Biomedical Sciences; Vol 5, No 1 (2021); 8-12
2527-3442
2527-4384
 
Language eng
 
Relation https://cellbiopharm.com/ojs/index.php/MCBS/article/view/166/67
 
Rights Copyright (c) 2021 sri wahdaningsih
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library