Variability of DNA based amplicon profiles generated by Bet v 1 homologous among different vegetable species

Acta Fytotechnica et Zootechnica

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Title Variability of DNA based amplicon profiles generated by Bet v 1 homologous among different vegetable species
Creator Zamieskova, Lucia
Žiarovská, Jana
Description Received: 2020-10-07 Accepted: 2021-02-08 Available online: 2021-02-28 10 is a group of proteins derived from various plant species that contains homologues of Bet v 1, majorbirch-pollen allergen, which are able to cross-react with an IgEs of sensitive patients and invoke allergic reactions. Despitenew sequencing technologies and the amount of data generated daily, PR-10 proteins have not been discovered in everyplant species. To describe genomic variability by the -omics sciences, it is necessary to screen an undiscovered members of thegroup, map ypr-10 genes in genome and determine sequence variabilities. In this study Bet v 1 based amplified profile (BBAP)method was used to analyse the variability of 14 vegetable species (Brassica oleracea in 4 varieties) and to compare generatedpolymorphism among them. For all of the analysed species, a total number of generated amplicons was 162. The most differentprofiles were generated by Pastinaca sativa and Capsicum annuum, the highest similarity was between Apium graveolens andB. oleracea var. capitata. New type of genic molecular markers was applied successfully and proved to be an effective techniqueto map variability of PR-10 group.Keywords: PR-10, Bet v 1 homologue, vegetable, DNA marker, cross-reaction, allergy, genic molecular markerReferencesANDERSEN, M.B.S. et al. (2009). Identification of European Allergy Patterns to the Allergen Families PR-10, LTP, and Profilin fromRosaceae Fruits. Clinic Rev Allerg Immunol, 41(1), 4–19.BREITENEDER, H. and EBNER, CH.M.B. (2000). Molecular and biochemical classification of plant-derived food allergens.Molecular Mechanisms in Allergy and Clinical Immunology, 106(1), P27–36.CABALLERO, T. and MARTÍN-ESTEBAN, M. (1998). Association between pollen hypersensitivity and edible vegetable allergy:a review. J Investig Allergol Clin Immunol., 8(1), 6–16.ELISYUTINA, O. et al. (2019). 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Application of genomic data for PCR screening of Bet v 1 conserved sequence inclinically relevant plant species. In: Systems Biology. London: IntechOpen, pp. 65–82. ISBN 978-1-83880-803-7.
Publisher Acta Fytotechnica et Zootechnica
Contributor Demand-driven research for the sustainable and innovative food, Drive4SIFood 313011V336
Date 2021-02-26
Type info:eu-repo/semantics/article

Format application/pdf
Source Acta Fytotechnica et Zootechnica; Vol 24 (2021): Actual Concepts in Agrobiology::Invited Editor: Ing. Jaroslav Andreji, PhD.
Language eng
Rights Copyright (c) 2021 Acta Fytotechnica et Zootechnica

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