Variability of DNA based amplicon profiles generated by Bet v 1 homologous among different vegetable species

Acta Fytotechnica et Zootechnica

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Title Variability of DNA based amplicon profiles generated by Bet v 1 homologous among different vegetable species
 
Creator Zamieskova, Lucia
Žiarovská, Jana
 
Description Received: 2020-10-07 Accepted: 2021-02-08 Available online: 2021-02-28https://doi.org/10.15414/afz.2021.24.mi-apa.1-6Pathogen-related 10 is a group of proteins derived from various plant species that contains homologues of Bet v 1, majorbirch-pollen allergen, which are able to cross-react with an IgEs of sensitive patients and invoke allergic reactions. Despitenew sequencing technologies and the amount of data generated daily, PR-10 proteins have not been discovered in everyplant species. To describe genomic variability by the -omics sciences, it is necessary to screen an undiscovered members of thegroup, map ypr-10 genes in genome and determine sequence variabilities. In this study Bet v 1 based amplified profile (BBAP)method was used to analyse the variability of 14 vegetable species (Brassica oleracea in 4 varieties) and to compare generatedpolymorphism among them. For all of the analysed species, a total number of generated amplicons was 162. The most differentprofiles were generated by Pastinaca sativa and Capsicum annuum, the highest similarity was between Apium graveolens andB. oleracea var. capitata. New type of genic molecular markers was applied successfully and proved to be an effective techniqueto map variability of PR-10 group.Keywords: PR-10, Bet v 1 homologue, vegetable, DNA marker, cross-reaction, allergy, genic molecular markerReferencesANDERSEN, M.B.S. et al. (2009). Identification of European Allergy Patterns to the Allergen Families PR-10, LTP, and Profilin fromRosaceae Fruits. Clinic Rev Allerg Immunol, 41(1), 4–19.BREITENEDER, H. and EBNER, CH.M.B. (2000). Molecular and biochemical classification of plant-derived food allergens.Molecular Mechanisms in Allergy and Clinical Immunology, 106(1), P27–36.CABALLERO, T. and MARTÍN-ESTEBAN, M. (1998). Association between pollen hypersensitivity and edible vegetable allergy:a review. J Investig Allergol Clin Immunol., 8(1), 6–16.ELISYUTINA, O. et al. (2019). 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Antagonistic effects of abscisic acid and jasmonates on salt stress-inducible transcripts in rice roots.Plant Cell, 9(12), 2243–2259.NIRGUDE et al. (2014). Development and molecular characterization of genic molecular markers for grain protein and calciumcontent in finger millet (Eleusine coracana (L.) Gaertn.). Mol Biol Rep, 41(3), 1189–1200.PARK, C.J. et al. (2004). Pathogenesis-related protein 10 isolated from hot pepper functions as a ribonuclease in an antiviralpathway. Plant J, 37(2), 186–198.PÜHRINGER, H. et al. (2000). The promoter of an apple Ypr10 gene, encoding the major allergen Mal d 1, is stress- and pathogeninducible.Plant Sci, 152(1), 35–50.ROBERT, N. et al. (2001). Molecular characterization of the incompatible interaction of Vitis vinifera leaves with Pseudomonassyringae pv. pisi: expression of genes coding for stilbene synthase and class 10 PR protein. Eur J Plant Pathol, 107(2), 249–261.UEHARA, M. et al. (2001). Sequential IgE epitope analysis of a birch pollen allergen (Bet v1) and an apple allergen (Mal d1).Allergology International, 50(1), 57–62.VARSHNEY R.K. (2010). Gene-based marker systems in plants: high throughput approaches for discovery and genotyping.In: Molecular techniques in crop improvement. The Netherlands: Springer, pp. 119–142.VARSHNEY et al. (2007). Genic molecular markers in plants: development and applications. In: Genomics-Assisted CropImprovement: vol. 1: Genomics Approaches and Platforms. Dordrecht: Springer, pp. 13–29.WHITE, T.J. et al. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR protocolsa guide to methods and applications. San Diego: Academic Press, pp. 315–322.WISNIEWSKI, M. et al. (2004). Distribution and partial characterization of seasonally expressed proteins in different aged shootsand roots of ‘Loring’ peach (Prunus persica). Tree Physiol, 24(3), 339–345.ŽIAROVSKÁ, J. and ZELEŇÁKOVÁ, L. (2016). Central and Eastern European spring pollen allergens and their expression analysisstateof the art. Diversity, 8(4), 1–11.ŽIAROVSKÁ, J. and ZELEŇÁKOVÁ, L. (2019). Application of genomic data for PCR screening of Bet v 1 conserved sequence inclinically relevant plant species. In: Systems Biology. London: IntechOpen, pp. 65–82. ISBN 978-1-83880-803-7.
 
Publisher Acta Fytotechnica et Zootechnica
 
Contributor Demand-driven research for the sustainable and innovative food, Drive4SIFood 313011V336
 
Date 2021-02-26
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://www.acta.fapz.uniag.sk/journal/index.php/on_line/article/view/788
 
Source Acta Fytotechnica et Zootechnica; Vol 24 (2021): Actual Concepts in Agrobiology::Invited Editor: Ing. Jaroslav Andreji, PhD.
1336-9245
1336-9245
 
Language eng
 
Relation http://www.acta.fapz.uniag.sk/journal/index.php/on_line/article/view/788/pdf
 
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