Genetic variability of commercially important apple varieties (Malus x domestica Borkh.) assessed by CDDP markers

Acta Fytotechnica et Zootechnica

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Title Genetic variability of commercially important apple varieties (Malus x domestica Borkh.) assessed by CDDP markers
Creator Bilčíková, Jana
Farkasová, Silvia
Žiarovská, Jana
Description  Received: 2020-10-13 Accepted: 2021-02-08 Available online: 2021-02-28 stand on the top of the most desirable and most produced fruit species in the world. Despite enormously wide geneticdiversity among existing apple varieties, the current market is mostly oriented on several cultivars of commercially attractivetraits. For the preservation of genetic resources and for breeding programmes, the evaluation of genetic diversity is fundamental.Number of marker systems have been developed and adopted in the assessment apple germplasm. In the present study, CDDPmarker technique was used to analyse polymorphism within the genomes of fifteen apple varieties which are of large commercialuse. Five primer combinations were used in the PCR amplification: WRKY-F1/WRKY-R1, WRKY-F1/WRKY-R2, WRKY-F1/WRKY-R2B,WRKY-F1/WRKY-R3 and WRKY-F1/WRKY-R3B. All primer combinations produced polymorphic amplification patterns. In someprimer combinations, identical amplification profiles were observed in few varieties, but amplification patterns of all combinationsmerged were specific for each apple variety. Identical profiles were only seen in Red Delicious and Granny Smith in F1/R1 primercombination, May Gold and Paula Red, and Selena and Melodie in F1/R2 primer combination and May Gold and Paula Red whenF1/R3B primers were used. Based on the CDDP markers, UPGMA algorithm assessed cultivars Paula Red and May Gold as themost similar (with similarity value of 0.909), whereas Gloster and Ambrosia showed to be the least similar within the analyzed set(similarity value of 0.200). The study proved CDDP markers to be suitable tool to produce polymorphic amplification patterns inapple genotypes.Keywords: conserved DNA-derived polymorphism, genetic variability, WRKY, DNA markersReferencesAouadi , M. et al. (2019). 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Publisher Acta Fytotechnica et Zootechnica
Date 2021-02-26
Type info:eu-repo/semantics/article

Format application/pdf
Source Acta Fytotechnica et Zootechnica; Vol 24 (2021): Actual Concepts in Agrobiology::Invited Editor: Ing. Jaroslav Andreji, PhD.
Language eng
Rights Copyright (c) 2021 Acta Fytotechnica et Zootechnica

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