In vitro evaluation of antileishmanial activity of copper (I) complexes

Acta Brasiliensis

View Publication Info
 
 
Field Value
 
Title In vitro evaluation of antileishmanial activity of copper (I) complexes

Avaliação in vitro da atividade antileishmania de complexos de cobre (I)
 
Creator Chagas, Ana Flávia da Silva
Porchia, Marina
Tisato, Francesco
Soldera, Pauline de Faria
Wyrepkowski, Claudia Dantas Comandolli
Naiff, Maricleide de Farias
Franco, Antonia Maria Ramos
 
Description In the research for the development of new drugs for the therapy of American tegumentary leishmaniasis, copper has been studied for its antileishmania activity. This study aims to report the activity of three copper(I) complexes on parasites of the species L. amazonensis and L. guyanensis. The metal complexes were tested according to in vitro antileishmanial assays, against promastigote and amastigote forms of the most prevalent species in the state of Amazonas, Brazil. Cytotoxicity of the complexes was evaluated in murine macrophage-like cell line (MJ774). The results of the in vitro assays indicated that, among the copper complexes tested, the homoleptic phosphine complex [Cu(thp)4][PF6](thp=tris-hydroxymethylphosphine) presented promising activity against the evolutionary forms of L. amazonensis, and obtained a IC50 of  26.45 and 24.61 µM in a period of 48 and 72 h, respectively. The results for copper complex at concentration 160 µM in amastigote forms showed a decrease in the infection index (32% of infected cells) and, in the cytotoxicity assay with MJ774, 52.43% of cell viability was observed. The results showed that the complex [Cu(thp)4][PF6] presented significant biological activity, indicating a need for future in vivo studies.
Na busca do desenvolvimento de novos fármacos para a terapia da leishmaniose tegumentar americana, o cobre tem sido estudado quanto a sua atividade antileishmania. Esse estudo tem como objetivo relatar a atividade de três complexos de cobre (I) sobre parasitas das espécies L. amazonensis e L. guyanensis. Os complexos metálicos foram testados por meio de ensaios antileishmania in vitro contra as formas promastigota e amastigota das espécies mais prevalentes no estado do Amazonas, Brasil. A citotoxicidade dos complexos foi avaliada em linhagem de células semelhantes a macrófagos murinos (MJ774). Os resultados dos ensaios in vitro indicaram que, entre os complexos de cobre testados, o complexo homoléptico de fosfina [Cu(thp)4][PF6](thp=tris-hidroximetilfosfina) apresentou atividade promissora contra as formas evolutivas de L. amazonensis, e obtiveram IC50 de 26,45 e 24,61 µM em um período de 48 e 72 h, respectivamente. Os resultados para o complexo de cobre na concentração de 160 µM nas formas amastigotas reportaram diminuição no índice de infecção (32% das células infectadas) e, no ensaio de citotoxicidade com MJ774, observou-se 52,43% de viabilidade celular. Os resultados evidenciaram que o complexo [Cu(thp)4][PF6] apresentou atividade biológica significativa, indicando a necessidade de futuros estudos in vivo.
 
Publisher Universidade Federal de Campina Grande - UFCG
 
Date 2021-01-27
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier http://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/474
10.22571/2526-4338474
 
Source Acta Brasiliensis; Vol 5 No 1 (2021): Acta Brasiliensis; 1-6
Acta Brasiliensis; Vol 5 No 1 (2021): Acta Brasiliensis; 1-6
Acta Brasiliensis; Vol 5 No 1 (2021): Acta Brasiliensis; 1-6
2526-4338
2526-432X
 
Language eng
 
Relation http://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/474/115
 
Rights Copyright (c) 2020 Acta Brasiliensis
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library