A STUDY ON INVOLVEMENT OF METAL-BINDING PROTEIN(S) FOR THE BIOSORPTION OF SOME SELECTED HEAVY METALS

JOURNAL OF ADVANCES IN BIOLOGY

View Publication Info
 
 
Field Value
 
Title A STUDY ON INVOLVEMENT OF METAL-BINDING PROTEIN(S) FOR THE BIOSORPTION OF SOME SELECTED HEAVY METALS
 
Creator Sarkar, Diptendu
Paul, Goutam
 
Subject Protease KExtracellular proteins; DSGPM4.
 
Description The present research finding have shown that extracellular protein(s) of growing cells of Pseudomonas aeruginosa DSGPM4 strain are responsible for giving withstand power in presence of  toxic heavy metals.  To study the role of cadmium, nickel and copper resistance, a preliminary test was done by treating the cells with different concentrations of proteinase K. To perform the test highly resistant isolates DSGPM4 was inoculated in nutrient broth and incubated overnight at 37⁰C. Reaction tubes were prepared by adding overnight cultures in two different concentrations of proteinase K i.e. 1 mg/ml and 2 mg/ml in the ratio of 1:1 and incubated for 2 hours at 37⁰C. After incubation, 20 ml of each sample was spread over selective plates containing two different concentrations of PbCl₂  and NiCl2 and CuCl2 i.e., 1mM and 2 mM in growth medium (1x A) as well as on control plates. Control plates were prepared by adding 1 mg/ml (C1), and 2 mg/ml (C2) of proteinase K whereas, other control plates containing 2mM of PbCl₂ (C3) and 2mM of NiCl2 (C4) and CuCl2 (C5) salts but no proteinase K.  To find out whether the extracellular protein has any role in metal resistance, preliminary test was conducted by treating the plasmid bearing isolate (DSGPM4) and a control DSGPM4 with protease.  This study reports the accumulation of some heavy metals (Cu, Ni and Pb) by growing cells of Pseudomonas aeruginosa DSGPM4 strain and the purification and molecular characterization of related heavy metal binding proteins.
 
Publisher CIRWORLD
 
Date 2014-09-06
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://cirworld.com/index.php/jab/article/view/5562
 
Source JOURNAL OF ADVANCES IN BIOLOGY; Vol 5 No 3; 692-696
2347-6893
 
Language eng
 
Relation http://cirworld.com/index.php/jab/article/view/5562/5373
 
Rights Copyright (c) 2017 JOURNAL OF ADVANCES IN BIOLOGY
http://creativecommons.org/licenses/by/4.0
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library