Laboratory Protocols for Isolation and Identification of Toxigenic Strains of Vibrio cholerae: A Review

International Journal for Research in Applied Sciences and Biotechnology

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Field Value
 
Title Laboratory Protocols for Isolation and Identification of Toxigenic Strains of Vibrio cholerae: A Review
 
Creator Kumar Shrestha, Bijay
Tumbahangphe, Manita
Shakya, Jenish
Chauhan, Sujata
Dhungana, Bidhya
Shrestha, Romika
Limbu, Jyoti
Khadka, Kabiraj
Rai, Anu
 
Subject Vibrio cholerae
Culture
Pathogenesis
Epidemiology
Faecal sample
food
sewage
water
PCR
 
Description Vibrio cholerae is a pathogenic bacteria that accounts for diarrheal illness which can be life-threatening in lack of treatment and accounts for great morbidity and mortality. The common mode of transmission includes fecal-oral route transmission mediated through contaminated food and water. The strains 01 and 0139 have been associated with epidemic outbreak of cholera with severe clinical manifestation whereas the infection caused by non O1 and non O139 strains are recognized as non-epidemic cholera characterized by mild-severe diarrheal syndrome without epidemic potential. Laboratory diagnosis through Culture and determining Antimicrobial susceptibility tests are recommended for diagnosis of cholera in all clinical laboratory settings. The simple conventional laboratory diagnosis through culture should be effective for diagnosis of cholera in the resource (PCR) limited laboratories. However, Vibrio cholerae in VBNC (Viable but non-culturable) state can only be detected through molecular methods. Therefore, molecular methods need to be extended in all epidemiological laboratories for preventing misidentification of Vibrio cholerae especially from environmental samples. Epidemic outbreak of cholera has explained the need of immediate preparedness with preventive, diagnosis and curative improvement in all health sectors. Therefore, immediate health and sanitation related awareness are necessary for prompt control of the disease before it leads community to global burden.
 
Publisher Vandana Publications
 
Date 2020-12-04
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://www.ijrasb.com/ojs/index.php/ojs-ijrasb/article/view/214
10.31033/ijrasb.7.6.26
 
Source International Journal for Research in Applied Sciences and Biotechnology; Vol. 7 No. 6 (2020): November Issue; 181-189
2349-8889
 
Language eng
 
Relation https://www.ijrasb.com/ojs/index.php/ojs-ijrasb/article/view/214/184
 
Rights Copyright (c) 2020 International Journal for Research in Applied Sciences and Biotechnology
https://creativecommons.org/licenses/by-nc-nd/4.0
 

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