A Comparative Study of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Fungal Culture for the Evaluation of Fungal Species in Patients with Tinea Cruris

Open Access Macedonian Journal of Medical Sciences

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Title A Comparative Study of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Fungal Culture for the Evaluation of Fungal Species in Patients with Tinea Cruris
 
Creator Hazlianda, Cut
Muis, Kamaliah
Lubis, Isma
 
Subject Tinea cruris
Dermatophyte
Culture
PCR-RFLP
Fungal species
Dermatology
 
Description BACKGROUND: Tinea cruris is the second most common dermatophytosis in the world and the most common in Indonesia. The conventional laboratory tests for dermatophyte infection are slow and less specific. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) is a PCR method with the addition of enzyme after amplification, therefore enabling for more specific results.AIM: This study aimed to find whether the PCR-RFLP test could yield the same fungal species result as a fungal culture.METHODS: The specimens were skin scrapings from thirty-one patients suspected tinea cruris. The tools and materials that were used were Sabaroud’s dextrose agar media, primer ITS 1 and ITS 4 and MvaI.RESULTS: The equation percentage of the test result species between PCR-RFLP and fungal culture was 50% of 12 subjects whose the test results were both positive from the fungal culture and PCR-RFLP. The percentage of the test result with fungal culture the fungal species were found, but in the PCR-RFLP test which the fungal species was not found, the percentage was 50% of 12 subjects which the test results were both positive as fungi from the culture and PCR-RFLP test.CONCLUSIONS: The species from PCR-RFLP examination was the same with the fungal culture.
 
Publisher Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia
 
Date 2017-11-21
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
The tools and materials that were used were Sabaroud’s dextrose agar media, primer ITS 1 and ITS 4 and MvaI.
 
Format application/pdf
application/pdf
 
Identifier https://www.id-press.eu/mjms/article/view/oamjms.2017.197
10.3889/oamjms.2017.197
 
Source Open Access Macedonian Journal of Medical Sciences; Vol. 5 No. 7 (2017): Dec 15 (OAMJMS); 844-847
1857-9655
 
Language eng
 
Relation https://www.id-press.eu/mjms/article/view/oamjms.2017.197/1691
https://www.id-press.eu/mjms/article/view/oamjms.2017.197/1658
 

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