Bacterial communities associated with apical periodontitis and dental implant failure

Microbial Ecology in Health and Disease

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Title Bacterial communities associated with apical periodontitis and dental implant failure
 
Creator Dingsdag, Simon; University of Sydney
Nelson, Stephen; University of Sydney
Coleman, Nicholas V.; University of Sydney
 
Subject Dental Microbiology; Apical Periodontitis; Alveolar Bacteria; Dental Implant Failure; T-RFLP; Pyrosequencing
 
Description Background: Previously, we demonstrated that bacteria reside in apparently healed alveolar bone, using culture and Sanger sequencing techniques. Bacteria in apparently healed alveolar bone may have a role in peri-implantitis and dental implant failure.Objective: To compare bacterial communities associated with apical periodontitis, those colonising a failed implant and alveolar bone with reference biofilm samples from healthy teeth.Methods and results: The study consisted of 196 samples collected from 40 patients undergoing routine dental implant insertion or rehabilitation. The bacterial 16S ribosomal DNA sequences were amplified. Samples yielding sufficient polymerase chain reaction product for further molecular analyses were subjected to terminal restriction fragment length polymorphism (T-RFLP; 31 samples) and next generation DNA sequencing (454 GS FLX Titanium; 8 samples). T-RFLP analysis revealed that the bacterial communities in diseased tissues were more similar to each other (p<0.049) than those from the healthy reference samples. Next generation sequencing detected 13 bacterial phyla and 373 putative bacterial species, revealing an increased abundance of Gram-negative [Prevotella, Fusobacterium (p<0.004), Treponema, Veillonellaceae, TG5 (Synergistetes)] bacteria and a decreased abundance of Gram-positive [(Actinomyces, Corynebacterium (p<0.008)] bacteria in the diseased tissue samples (n=5) relative to reference supragingival healthy samples (n=3).Conclusion: Increased abundances of Prevotella, Fusobacterium and TG5 (Synergistetes) were associated with apical periodontitis and a failed implant. A larger sample set is needed to confirm these trends and to better define the processes of bacterial pathogenesis in implant failure and apical periodontitis. The application of combined culture-based, microscopic and molecular technique-based approaches is suggested for future studies.Keywords: oral bacterial communities; apical periodontitis; alveolar bone; dental implant failure; T-RFLP; pyrosequencing(Published: 8 November 2016)Citation: Microbial Ecology in Health & Disease 2016, 27: 31307 - http://dx.doi.org/10.3402/mehd.v27.31307
 
Publisher Microbial Ecology in Health and Disease
 
Contributor
 
Date 2016-11-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
text/html
application/epub+zip
application/xml
 
Identifier http://www.microbecolhealthdis.net/index.php/mehd/article/view/31307
10.3402/mehd.v27.31307
 
Source Microbial Ecology in Health and Disease; Vol 27 (2016)
1651-2235
 
Language eng
 
Relation http://www.microbecolhealthdis.net/index.php/mehd/article/view/31307/49767
http://www.microbecolhealthdis.net/index.php/mehd/article/view/31307/49768
http://www.microbecolhealthdis.net/index.php/mehd/article/view/31307/49769
http://www.microbecolhealthdis.net/index.php/mehd/article/view/31307/49770
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/31307/26353
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/31307/26355
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/31307/29545
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/31307/29546
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/31307/32031
 
Rights Copyright (c) 2016 Simon Dingsdag, Stephen Nelson, Nicholas V Coleman
http://creativecommons.org/licenses/by-nc/4.0
 

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