HSPA8, not C3AR1 trapped from SH-SY5Y cells: demonstrated by TLQP-21 avidin agarose affinity chromatography: HSPA8, not C3AR1 trapped by TLQP-21 in SH-SY5Y cells

To Chemistry Journal

View Publication Info
 
 
Field Value
 
Title HSPA8, not C3AR1 trapped from SH-SY5Y cells: demonstrated by TLQP-21 avidin agarose affinity chromatography: HSPA8, not C3AR1 trapped by TLQP-21 in SH-SY5Y cells
 
Creator Md. Shamim Akhter
Jesus Rodriguez Requena
 
Subject SH-SY5Y cells
Avidin agarose affinity chromatography
TLQP-21
C3AR1
HSPA8
Molecular Medicine
 
Description To ‘fish out’ HSPA8 as a receptor of human TLQP-21 in human neuroblastoma SH-SY5Y cell; cross-linking, affinity chromatography and mass spectrometry-based protein identification techniques were used (by our group). Before that, in rodent cells, C3AR1 was found as a receptor of rodent TLQP-21 in CHO-K1 cells (by another group). So, it was of very much interest to find out whether C3AR1 can be ‘fished out’ as a receptor of human TLQP-21 in a human SH-SY5Y cell line or not. Here PVDF membrane (which was used for immunohistochemical validation of HSPA8 in SH-SY5Y cell line) was stripped, followed by immunohistochemical validation as before under the same condition, but C3AR1 was not found to bind with human TLQP-21. The findings concluded here that in SH-SY5Y cells, HSPA8 but not C3AR1 was documented as a receptor of human TLQP-21 using avidin agarose affinity chromatography. The significance of the study is that it provides a starting point to signpost: human TLQP-21 exerts its biological activity via HSPA8 (not C3AR1) in human SH-SY5Y cells. The further readout of TLQP-21-HSPA8 signaling can be exploited to explore new horizon in diagnosis and therapies for VGF related human diseases, especially in which TLQP-21 has been shown to affect and related with thereof.
 
Publisher PURKH
 
Date 2020-08-15
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://purkh.com/index.php/tochem/article/view/746
 
Source To Chemistry Journal; Vol. 6: May - August 2020; 125-131
2581-7507
 
Language eng
 
Relation https://purkh.com/index.php/tochem/article/view/746/777
 
Rights Copyright (c) 2020 Md. Shamim Akhter , Jesus Rodriguez Requena
https://creativecommons.org/licenses/by/4.0
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library