Microbial culturomics unravels the halophilic microbiota repertoire of table salt: description of Gracilibacillus massiliensis sp. nov.

Microbial Ecology in Health and Disease

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Title Microbial culturomics unravels the halophilic microbiota repertoire of table salt: description of Gracilibacillus massiliensis sp. nov.
 
Creator Diop, Awa; URMITE, UMR CNRS 6236-IRD 198, Faculté de Medecine, Aix-Marseille Université, Bouches-du-Rhône
Khelaifia, Saber; URMITE, UMR CNRS 6236-IRD 198, Faculté de Medecine, Aix-Marseille Université, Bouches-du-Rhône
Armstrong, Nicholas; URMITE, UMR CNRS 6236-IRD 198, Faculté de Medecine, Aix-Marseille Université, Bouches-du-Rhône
Labas, Noémie; URMITE, UMR CNRS 6236-IRD 198, Faculté de Medecine, Aix-Marseille Université, Bouches-du-Rhône
Fournier, Pierre-Edouard; URMITE, UMR CNRS 6236-IRD 198, Faculté de Medecine, Aix-Marseille Université, Bouches-du-Rhône
Raoult, Didier; URMITE, UMR CNRS 6236-IRD 198, Faculté de Medecine, Aix-Marseille Université, Bouches-du-Rhône
Million, Matthieu; URMITE, UMR CNRS 6236-IRD 198, Faculté de Medecine, Aix-Marseille Université, Bouches-du-Rhône
 
Subject bacteria; microbial communities; microbiology of unexplored habitats; extremophiles/extremophily; uncultured microbes; microbial ecology
 
Description Background: Microbial culturomics represents an ongoing revolution in the characterization of environmental and human microbiome.Methods: By using three media containing high salt concentration (100, 150, and 200 g/L), the halophilic microbial culturome of a commercial table salt was determined.Results: Eighteen species belonging to the Terrabacteria group were isolated including eight moderate halophilic and 10 halotolerant bacteria. Gracilibacillus massiliensis sp. nov., type strain Awa-1T (=CSUR P1441=DSM 29726), is a moderately halophilic gram-positive, non-spore-forming rod, and is motile by using a flagellum. Strain Awa-1T shows catalase activity but no oxidase activity. It is not only an aerobic bacterium but also able to grow in anaerobic and microaerophilic atmospheres. The draft genome of G. massiliensis is 4,207,226 bp long, composed of 13 scaffolds with 36.05% of G+C content. It contains 3,908 genes (3,839 protein-coding and 69 RNA genes). At least 1,983 (52%) orthologous proteins were not shared with the closest phylogenetic species. Hundred twenty-six genes (3.3%) were identified as ORFans.Conclusions: Microbial culturomics can dramatically improve the characterization of the food and environmental microbiota repertoire, deciphering new bacterial species and new genes. Further studies will clarify the geographic specificity and the putative role of these new microbes and their related functional genetic content in environment, health, and disease.Keywords: Gracilibacillus massiliensis; taxono-genomics; culturomics; microbial community; salt; halophile(Published: 18 October 2016)Citation: Microbial Ecology in Health & Disease 2016, 27: 32049 - http://dx.doi.org/10.3402/mehd.v27.32049
 
Publisher Microbial Ecology in Health and Disease
 
Contributor “Fondation Méditerranée Infection”.
 
Date 2016-10-19
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
text/html
application/epub+zip
application/xml
 
Identifier http://www.microbecolhealthdis.net/index.php/mehd/article/view/32049
10.3402/mehd.v27.32049
 
Source Microbial Ecology in Health and Disease; Vol 27 (2016)
1651-2235
 
Language eng
 
Relation http://www.microbecolhealthdis.net/index.php/mehd/article/view/32049/49241
http://www.microbecolhealthdis.net/index.php/mehd/article/view/32049/49242
http://www.microbecolhealthdis.net/index.php/mehd/article/view/32049/49243
http://www.microbecolhealthdis.net/index.php/mehd/article/view/32049/49244
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/32049/27904
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/32049/27905
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/32049/27906
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/32049/27907
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/32049/27908
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/32049/27909
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/32049/27910
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/32049/27911
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/32049/27912
 
Rights Copyright (c) 2016 Awa DIOP, Saber KHELAIFIA, Nicholas ARMSTRONG, Noémie LABAS, Pierre-Edouard FOURNIER, Didier RAOULT, Matthieu MILLION
http://creativecommons.org/licenses/by-nc/4.0
 

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