The Role of adaptive MSCs as an Attempt Regeneration of Spermatogenesis Process Using by Hypoxia Precondition In Vitro

Journal of Stem Cell Research and Tissue Engineering

View Publication Info
 
 
Field Value
 
Title The Role of adaptive MSCs as an Attempt Regeneration of Spermatogenesis Process Using by Hypoxia Precondition In Vitro
 
Creator safitri, erma
 
Subject stem cell, medicine
MSCs, hypoxia precondition 3% O2 concentration, adaptive.
 
Description The aim of this research was to obtain to get adaptive MSCs by an treatment in hypoxia precondition in viro culture. Ini this research, hypoxia precondition was to be given 3% O2 concentration and compared to those of normoxia culture in O2 > 20%. Results showed that under 3% O2 concentration, CD105+ and CD45- by flowcytometri, immunocytokimia and immunofluorescence didn’t experience of change (undifferentiated). Meanwhile under > 20% O2 concentration, cells have experienced of change (not undifferentiated again), that was indicated with CD105+ become decrease and CD45- increase by flowcytometri, immunocytokimia and immunofluorescence. Conclusion, in this research showed that hypoxia precondition with 3% O2 concentration very support MSCs to constantly adaptive before transplantated for disturbance of spermatogenesis process, because did’nt experience become progenitor cells was not expectation (still undifferentiated).
 
Publisher Stem Cell Research and Development Center, Universitas Airlangga
 
Contributor
 
Date 2021-01-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://e-journal.unair.ac.id/JSCRTE/article/view/22752
10.20473/jscrte.v4i2.22752
 
Source Journal of Stem Cell Research and Tissue Engineering; Vol 4, No 2 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING; 64-69
2614-1256
2614-1264
 
Language eng
 
Relation https://e-journal.unair.ac.id/JSCRTE/article/view/22752/12412
 
Rights Copyright (c) 2020 Journal of Stem Cell Research and Tissue Engineering
http://creativecommons.org/licenses/by-nc-sa/4.0
 

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