The Effect of Bone Marrow Transplantation on Oocyte-Granulosa Cell Interaction and Follicular Development of Cisplatin-Induced Ovarian Failure in Rat

Journal of Stem Cell Research and Tissue Engineering

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Title The Effect of Bone Marrow Transplantation on Oocyte-Granulosa Cell Interaction and Follicular Development of Cisplatin-Induced Ovarian Failure in Rat
 
Creator hendarto, hendi
 
Subject stem cell, medicine
Bone Marrow, GDF-9, Kit-ligand, follicle, Ovarian failure
 
Description Introduction: Chemotherapy has cytotoxic effect that induces follicular damage and abnormal folliculogenesis leads to ovarian failure. Two crucial   growth factors in abnormal folliculogenesis, Growth Differentiation Factor-9 (GDF-9) and Kit-Ligand, will be disrupted and affect follicular development. In this study, we evaluate whether bone marrow transplantation (BMT) has a role on oocyte-granulosa cell interaction by analyzing GDF-9 and Kit-Ligand expressions and also follicular development by analyzing primordial, primary, secondary and graafian follicles of cisplatin-induced ovarian failure in rat. Material and method: Forty eight rats were divided into three groups: control, cisplatin and cisplatin + BMT. Ovarian failure was induced by administration   of intraperitoneal cisplatin 5 mg/kg body weight for 1 week. BMT 2 x107 cells were injected through rat tail vein after cisplatin administration.  Bone marrow was isolated from rat femur and characterized    by CD44(+), CD45(-), CD105(+). Immunohistochemistry examinations for ovarian GDF-9, Kit-Ligand and follicle development evaluation were performed after 2 weeks of BMT injection. Results:  The  expressions   of Kit-ligand  among  three  groups  by ANOVA were  significant different (p=0.00), whereas by Post Hoc: cisplatin group lower  than  control  group (p=0.00); cisplatin + BMT group  higher than  cisplatin group (p=0.00); and no significant different between  control  group and cisplatin + BMT group (p=0.955). The expressions of GDF-9 by Kruskal Wallis showed significant different (p=0.00) among three groups whereas cisplatin + BMT group higher than cisplatin group and control group. In cisplatin + BMT group the number of primordial, primary, secondary and graafian follicles were higher than those in cisplatin group; but were lower than those in control group (p=0.000). Positive Paul Kart Horan (PKH) labeling was seen in cisplatin + BMT   group only. Conclusion:  In cisplatin-induced ovarian failure in rat, bone marrow transplantation may improve oocytegranulosa cell interaction and follicular development.
 
Publisher Stem Cell Research and Development Center, Universitas Airlangga
 
Contributor
 
Date 2021-01-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://e-journal.unair.ac.id/JSCRTE/article/view/22751
10.20473/jscrte.v4i2.22751
 
Source Journal of Stem Cell Research and Tissue Engineering; Vol 4, No 2 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING; 56-63
2614-1256
2614-1264
 
Language eng
 
Relation https://e-journal.unair.ac.id/JSCRTE/article/view/22751/12410
 
Rights Copyright (c) 2020 Journal of Stem Cell Research and Tissue Engineering
http://creativecommons.org/licenses/by-nc-sa/4.0
 

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