Optimation of Hematopoietic Stem Cells’ Culture Medium: A Review

Journal of Stem Cell Research and Tissue Engineering

View Publication Info
 
 
Field Value
 
Title Optimation of Hematopoietic Stem Cells’ Culture Medium: A Review
 
Creator prasetyadi, prasetyadi
 
Subject medicine
Hematopoietic stem cells, culture medium supplementation, CD34+
 
Description Hematopoietic stem cells (HSCs) usage has numerous potential benefits for basic and clinical research.  Growing interest in this field is caused by possible HSCs application   to cure   various   hematologic   diseases, malignancies, immunodeficiency diseases, and inborn errors of metabolism.  Although the demand of HSCs is increasing, ex vivo culture of HSCs has various medium compositions. This article focuses on HSCs’ culture medium optimation in different medium supplements, which might overcome non-optimal HSCs culture results. Literature searching was conducted in PubMed and google scholar database. Paper selection processes were done by author separately and studies which met the eligible criteria were included in this review. A total of 53 relevant articles were identified and 11 articles that met the eligible criteria were included in this review. There are two main types of supplement, cytokines and non-cytokines.  In cytokines addition, 7 studies generally show that the supplement support HSCs expansion.  The addition of non-cytokines supplements has more diverse   result in studies.   Positive   results   with various   effectivity   in CD34+ expansion were shown in 3 studies, while one study shows a negative result. Medium supplementation must be explored more to find best substances which can optimize   HSCs   culture.   Further   research   using   non-cytokines   substance   shall   be conducted regarding its various effect and might give us a big opportunity to find an optimal condition for HSCs culture.
 
Publisher Stem Cell Research and Development Center, Universitas Airlangga
 
Contributor
 
Date 2020-06-30
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://e-journal.unair.ac.id/JSCRTE/article/view/20129
10.20473/jscrte.v3i2.20129
 
Source Journal of Stem Cell Research and Tissue Engineering; Vol 3, No 2 (2019): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING; 41-46
2614-1256
2614-1264
 
Language eng
 
Relation https://e-journal.unair.ac.id/JSCRTE/article/view/20129/11349
 
Rights Copyright (c) 2020 Journal of Stem Cell Research and Tissue Engineering
http://creativecommons.org/licenses/by-nc-sa/4.0
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library