Characterization of CD4+ Lymphocyte From Bone Marrow Stem Cell Using Indirect Immunofluoresence For HIV & AIDS Treatment

Journal of Stem Cell Research and Tissue Engineering

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Field Value
 
Title Characterization of CD4+ Lymphocyte From Bone Marrow Stem Cell Using Indirect Immunofluoresence For HIV & AIDS Treatment
 
Creator Purwati, Purwati
 
Subject stem cell, medicine
characterization, Lymphocyte T CD4, HIV & AIDS.
 
Description Acquired immune deficiency syndrome (AIDS) is caused by Human Immunodeficiency Virus (HN). At the beginning of infection, gpl20 virus interacts with CD4 receptor at the surface of the target cell. The interaction between gpI20 and CD4 leads to the occurrence of the binding of specific chemokine receptor CXCR4 and CCR5, which are also present on the membrane of the target cell.  Therefore, CCR5 arid CXCR4 also determine the fate of the target cell.  It is the performance   of CCR5 and CXCR4, guided by controlling gene that determines susceptibility or resistance to HN infection.  Coding gene CCR5 may mutate to become protective or resistant against HTV infection. In homozygote   individuals, it tends to be resistant against infection while in heterozygote individuals it tends to be susceptible to HN infection.  Objective:  To characterize   TCD4 lymphocyte in the next that is resistant against HN infection by using gene therapy deletion 32 CCR5 to use for HTV & AIDS treatment. Method: Sample collection, mononucleated cell collection, lymphocyte culture, CD4 identification, CCR5 variance analysis, co-cultivation with PBMC HN and comparison to control. Result:  This study was performed in several steps, such as mononucleated   cell isolation, followed with cell culture, lymphocyte purification, lymphocyte and CD4 expression identification.   Conclusion:  Lymphocyte T CD4 had been mature after seven passages, once passage is about 5 days so for maturity lymphocyte T CD4 need 35 days and that cell as be candidate to resistant against HN infection by using gene therapy deletion 32 CCR5 to use for HN   & AIDS treatment.
 
Publisher Stem Cell Research and Development Center, Universitas Airlangga
 
Contributor
 
Date 2021-01-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://e-journal.unair.ac.id/JSCRTE/article/view/22749
10.20473/jscrte.v4i2.22749
 
Source Journal of Stem Cell Research and Tissue Engineering; Vol 4, No 2 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING; 49-55
2614-1256
2614-1264
 
Language eng
 
Relation https://e-journal.unair.ac.id/JSCRTE/article/view/22749/12409
 
Rights Copyright (c) 2020 Journal of Stem Cell Research and Tissue Engineering
http://creativecommons.org/licenses/by-nc-sa/4.0
 

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