Pengaruh Cercospora sp. terhadap Kandungan Asam Askorbat pada Mekanisme Patogenisitas Bercak Daun Tanaman Cabai : Kajian secara In Vitro dan In Planta

BioEksakta : Jurnal Ilmiah Biologi Unsoed

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Title Pengaruh Cercospora sp. terhadap Kandungan Asam Askorbat pada Mekanisme Patogenisitas Bercak Daun Tanaman Cabai : Kajian secara In Vitro dan In Planta
Pengaruh Cercospora sp. terhadap Kandungan Asam Askorbat pada MekanismePatogenisitas Bercak Daun Tanaman Cabai : Kajian secara In vitro dan In planta
 
Creator Yuliawati, Nasriyatun
Mumpuni, Aris
Muljowati, Juni Safitri
 
Description Red chili is a vegetable commodity that has high economic value in Indonesia. Leaf spot disease caused by the fungus Cercospora sp. is one of the limiting factors in red chili production. The occurrence of leaf spot disease is determined by the success of the pathogenesis by the fungus Cercospora sp. In addition, red chilies that are resistant to leaf spot disease have higher ascorbic acid content than vulnerable red chilies. The purpose of this study was to determine the ability to grow pathogens Cercospora sp. on the medium which was given ascorbic acid and know the effect of inoculation of the pathogen Cercospora sp. against ascorbic acid content in red chili leaves (C. annuum L.). This study used an experimental design with a completely randomized design (CRD). In vitro tests carried out consisted of PDA and PDB medium which were given ascorbic acid with a concentration of 0 mg.l-1, 0,25 mg.l-1, 0,5 mg.l-1, 0,75 mg.l-1 and 1,0 mg.l-1. In planta testing was using hot chili red chili varieties, large red chili varieties and curly red chili varieties. The treatments that were tested included calculation of disease intensity and ascorbic acid content in red chili leaves. In vitro test the main parameters observed were the diameter colony of the fungus Cercospora sp. dan mycelium dry weight. In planta test the main parameters observed were the intensity of the disease, while the supporting parameters were the incubation period of the disease, the content of ascorbic acid in the red chili leaves, temperature and humidity. In vitro test data obtained were analyzed using Analysis of Variance (ANOVA) at a 95% confidence level, then the treatment that gave a real or very real difference was followed by the Least Significant Difference test (LSD). In planta test data obtained were analyzed using Analysis of Variance (ANOVA) at a 95% confidence level, then the treatment that gave a real or very real difference was followed by the Least Significant Difference test (LSD). The results showed that the pathogen Cercospora sp. able to grow well on the PDA medium and GDP medium which were given ascorbic acid. Inoculation of pathogen Cercospora sp. can increase ascorbic acid content in red chili leaves.
 
 
Cabai merah merupakan salah satu komoditas sayuran yang bernilai ekonomi tinggi di Indonesia. Penyakit bercak daun yang disebabkan oleh jamur Cercospora sp. merupakan salah satu faktor pembatas produksi cabai merah. Terjadinya penyakit bercak daun ditentukan oleh keberhasilan patogenesis oleh jamur Cercospora sp. Selain itu, cabai merah yang tahan terhadap penyakit bercak daun memiliki kandungan asam askorbat lebih tinggi daripada cabai merah yang rentan. Tujuan penelitian ini adalah mengetahui kemampuan tumbuh patogen Cercospora sp. pada medium yang diberi asam askorbat dan mengetahui pengaruh inokulasi patogen Cercospora sp. terhadap kandungan asam askorbat pada daun cabai merah (C. annuum L.). Penelitian ini menggunakan eksperimental dengan Rancangan Acak Lengkap (RAL). Uji secara in vitro percobaan yang dilakukan terdiri atas medium PDA maupun PDB yang diberi asam askorbat dengan konsentrasi 0 mg.l-1, 0,25 mg.l-1, 0,5 mg.l-1, 0,75 mg.l-1 dan 1,0 mg.l-1. Uji secara in planta yaitu menggunakan varietas cabai merah hot chili, varietas cabai merah besar dan varietas cabai merah keriting. Perlakuan yang dicobakan meliputi perhitungan intensitas penyakit dan kandungan asam askorbat pada daun cabai merah. Uji in vitro parameter utama yang diamati yaitu diameter koloni jamur Cercospora sp., dan bobot kering miselium. Uji in planta parameter utama yang diamati yaitu intensitas penyakit, sedangkan parameter pendukung yaitu periode inkubasi penyakit, kandungan asam askorbat pada daun cabai merah, temperatur dan kelembapan udara. Data uji in vitro yang diperoleh dianalisis menggunakan Analysis of Variance (ANOVA) pada tingkat kepercayaan 95%, kemudian perlakuan yang memberikan perbedaan nyata atau sangat nyata dilanjutkan dengan uji Beda Nyata Terkecil (BNT). Data uji in planta yang diperoleh dianalisis menggunakan Analysis of Variance (ANOVA) pada tingkat kepercayaan 95%, kemudian perlakuan yang memberikan perbedaan nyata atau sangat nyata dilanjutkan dengan uji Beda Nyata Jujur (BNJ). Hasil penelitian menunjukkan bahwa patogen Cercospora sp. mampu tumbuh dengan baik pada medium PDA maupun medium PDB yang diberi asam askorbat. Inokulasi patogen Cercospora sp. dapat meningkatkan kandungan asam askorbat pada daun cabai merah.
 
 
 
Publisher Fakultas Biologi Universitas Jenderal Soedirman
 
Date 2020-07-28
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://jos.unsoed.ac.id/index.php/bioe/article/view/1896
10.20884/1.bioe.2020.2.2.1896
 
Source BioEksakta : Jurnal Ilmiah Biologi Unsoed; Vol 2 No 2 (2020): BioEksakta; 280-287
2714-8564
 
Language eng
 
Relation http://jos.unsoed.ac.id/index.php/bioe/article/view/1896/1756
 
Rights Copyright (c) 2020 BioEksakta
 

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