PENGARUH MEDIA TERKONDISI SEL PUNCA MESENSIMAL TERHADAP EKSPRESI GEN TRANSCRIPTION FACTOR 7-LIKE 2 (TCF7L2) TIKUS MODEL DIABETES MELITUS TIPE 2

Berita Biologi

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Title PENGARUH MEDIA TERKONDISI SEL PUNCA MESENSIMAL TERHADAP EKSPRESI GEN TRANSCRIPTION FACTOR 7-LIKE 2 (TCF7L2) TIKUS MODEL DIABETES MELITUS TIPE 2
 
Creator Widhiastuti, Stefani Santi
Branitamahisi, Bernadia
Inayati, Nor Sri
Preharsini, Ida Ayu
Handika, Demas Bayu
Sadewa, Ahmad Hamim
Laqif, Abdurahman
Haryana, Sofia Mubarika
 
Subject Conditioned medium, Diabetes mellitus, HOMA-β, TCF7L2
 
Description Diabetes mellitus type 2 is the most common type of diabetes. This study was conducted to determine the effect of Mesenchymal Stem Cell-Conditioned Medium (MSC-CM) in Homeostatic Model Assessment of β-cell function (HOMA-β) value, normal Langerhans cells, and Transcription Factor 7-Like 2 (TCF7L2) gene expression in type 2 diabetic rats model. As many as 27 male Sprague Dawley rats were divided into 3 study research groups: normal control (9 normal rats), diabetic control (9 type 2 diabetic rats, induced by 60 mg/kg BW Streptozotocin and 120 mg/kg BW Nicotinamide i.p.), and treatment (9 type 2 diabetic rats treatment with 0.1 ml/200g BW MSC-CM i.p.). On day 30 after therapy, the expression of TCF7L2 gene was performed with real time-quantitative PCR (RT-qPCR). The HOMA-β value were calculated based on Fasting Insulins (FINs) levels and Fasting Blood Glucose (FBG) levels data from other research team members. Based on results, MSC-CM increases the HOMA-β value and amount of normal Langerhans cells of treatment group that indicates amelioration effect of MSC-CM, but there was no significant difference in TCF7L2 gene expression level between diabetic control and treatment group.  
 
Publisher Research Center for Biology-Indonesian Institute of Sciences
 
Contributor
 
Date 2020-08-28
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/3830
10.14203/beritabiologi.v19i2.3830
 
Source BERITA BIOLOGI; Vol 19, No 2 (2020); 141 – 150
BERITA BIOLOGI; Vol 19, No 2 (2020); 141 – 150
2337-8751
0126-1754
 
Language eng
 
Relation http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/3830/pdf
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/downloadSuppFile/3830/330
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/downloadSuppFile/3830/331
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/downloadSuppFile/3830/332
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/downloadSuppFile/3830/333
 
Rights Copyright (c) 2020 BERITA BIOLOGI
 

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