Leaf anatomy of Conyza sumatrensis byotipes susceptible and resistant to glyphosate

Agrarian

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Title Leaf anatomy of Conyza sumatrensis byotipes susceptible and resistant to glyphosate
Anatomia foliar de biótipos de Conyza sumatrensis suscetível e resistente ao glifosato
 
Creator Schneider, Theodoro
Favaretto, Adriana
Rizzardi, Mauro Antônio
 
Subject
Anatomic characterization. Horseweed. Vascular bundles. Epidermalthickness. Stomatal index.
Ciências Agrárias; Agronomia; Fitotecnia
Caracterização anatômica. Buva. Feixes vasculares. Espessura da epiderme. Índice estomático.
 
Description Conyza sumatrensis is a weed that have resistance to the herbicide glyphosate. Resistance may be involved with anatomical differences between biotypes resistant and susceptible to the herbicide. The objective of this work was to perform the anatomical description of the leaf of Conyza sumatrensis resistant and susceptible to the herbicide glyphosate, with a view to identify possible alterations that may alter the absorption and/or translocation of the herbicide. For this, leaves of the resistant biotypes and one susceptible to glyphosate were collected and fixed in FAA 70 and later fixed in ethanol 70. The determination of the thickness of the epidermis on the adaxial side of the leaves of the biotypes, diameter of the vascular bundles and density and stomatal index were performed. The histological analyzes were performed using an optical microscope, and photographs of at least four readings were taken on each slide. The results observed in the characterization of the adaxial epidermis and the diameter of the bundles not showed differences between resistant and susceptible biotypes. On the other hand, the susceptible biotype presents higher density and stomatal index when compared to the others, explaining the difference of susceptibility to the glyphosate herbicide among the materials.
Conyza sumatrensis é uma planta daninha que apresenta resistência ao herbicida glifosato. A resistência pode ser devido a diferenças anatômicas entre biótipos resistentes e suscetíveis ao herbicida. O objetivo deste trabalho foi realizar a descrição anatômica da folha de biótipos de Conyza sumatrensis resistentes e suscetível ao herbicida glifosato, com vistas a identificar possíveis alterações que possam influenciar a absorção e/ou a translocação do herbicida. Para tanto, foram coletadas folhas de dois biótipos resistentes e um suscetível ao glifosato e fixadas em FAA 70 e, posteriormente, em etanol 70. Foram realizadas a determinação da espessura da epiderme na face adaxial das folhas, diâmetro dos feixes vasculares, além do índice e densidade estomática. As análises histológicas foram realizadas utilizando-se microscópio óptico, sendo tomadas fotografias de, no mínimo, quatro leituras em cada lâmina. Os resultados observados na caracterização da epiderme adaxial e do diâmetro dos feixes vasculares, não apresentaram diferenças entre os biótipos resistente e suscetível. Por outro lado, o biótipo suscetível apresenta maior densidade e índice estomático quando comparado aos demais, podendo estar relacionado com a suscetibilidade ao herbicida glifosato entre os materiais.
 
Publisher Editora UFGD
 
Contributor

 
Date 2020-07-27
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion


 
Format application/pdf
 
Identifier https://ojs.ufgd.edu.br/index.php/agrarian/article/view/9536
10.30612/agrarian.v13i49.9536
 
Source Agrarian Journal; v. 13, n. 49 (2020); 330-338
Agrarian; v. 13, n. 49 (2020); 330-338
1984-2538
 
Language por
 
Relation https://ojs.ufgd.edu.br/index.php/agrarian/article/view/9536/5906
https://ojs.ufgd.edu.br/index.php/agrarian/article/downloadSuppFile/9536/1807
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Rights Direitos autorais 2020 Agrarian
https://creativecommons.org/licenses/by-nc-sa/3.0/br/
 

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