Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique

Journal of Parasite Science

View Publication Info
 
 
Field Value
 
Title Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique
 
Creator Fanani, Firman Hadi
Kusnoto, Kusnoto
Hastutiek, Poedji
Yunus, Muchammad
Koesdarto, Setiawan
Suprihati, Endang
 
Subject
Haemonchus contortus, Fasciola gigantica, Cross reaction, WesternBlot
 
Description The purpose of this research to know cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum by using the Western Blot technique. The result can be used as Haemonchosis serologic diagnostic. This research was conducted using Haemonchus contortus worms obtained from abomasum, especially cow worms and Fasciola gigantica worms obtained from the bile duct and gall bladder. The worms were crushed and added with Phosphate Buffer Saline (PBS) to make homogenates and then centrifuged at 3500 rpm for 15 minutes. Homogenate Fasciola gigantica was immunized to mice to get anti-Fasciola gigantica serum at a dose of 200 μg/mice and booster 3 times with a span of two weeks. Homogenates were carried out using the SDS-PAGE technique to analyze proteins using a brilliant blue dye. Cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum byusing Western blot technique and obtained 3 protein bands are 94.9, 52.7, 46.7 kDa.
 
Publisher Universitas Airlangga
 
Contributor
 
Date 2020-06-27
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier https://e-journal.unair.ac.id/JoPS/article/view/20176
10.20473/jops.v4i1.20176
 
Source Journal of Parasite Science; Vol 4, No 1 (2020): Journal of Parasite Science; 1-6
2656-5331
2599-0993
 
Language eng
 
Relation https://e-journal.unair.ac.id/JoPS/article/view/20176/11152
 
Rights Copyright (c) 2020 Journal of Parasite Science
http://creativecommons.org/licenses/by-nc-sa/4.0
 

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