The Development, Verification and Evaluation of Container Closure Integrity Assessment of Prefilled Syringes Using Fluorescence Spectroflurometer

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Field Value
 
Title The Development, Verification and Evaluation of Container Closure Integrity Assessment of Prefilled Syringes Using Fluorescence Spectroflurometer
 
Creator Shripad Deshpande
Deepak D. Kayande
Mazahar Farooqui
Parag Deshmukh
 
Subject Prefilled Syringes
Container Closure System
Methylene Blue
Fluorescence-UV Detection
Analytical Chemistry
 
Description The preference of Prefilled syringes is increased over all the vials as container closure systems for liquid injections, when facilitated or self-administration is required.However, prefilled syringes are more complex compared to container closure system(CCS) consisting of vial, rubber stopper and flip off. Container closure integrity assurance and verification has been a specific challenge for prefilled syringes as they feature several sealing areas. A comprehensive understanding of the container closure system is necessary for an appropriate container closure integrity assessment as well as for packagingdevelopment and qualification.
Method for the measurement ofcontainer closure integrity(CCI) of prefilled syringes using fluorescence spectrophotometry was developed and validated with a spectroflurometer.Methylene blue solution was initially evaluated as the fluorophore ina syringe with excitation at 605 nm and emission at 678 nm, which generated a limit of detection of 0.06µg/mL. Furtherstudies were conducted using Rhodamine 123, a dye with stronger fluorescence. Using 482 nm excitation and 527 nmemission, the dye in the syringe could be easily detected at levels as low as 0.001 µg/mL. The relative standard deviationfor sixmeasurements of three different sample with different concentration was less than 2.8%. A number of operational parameters were optimized, including the photomultiplier tube voltage, excitation, andemission slit widths. The specificity of container closure integrity was checked by using marketed drug products sample,which showed no interference to the rhodamine detection. Results obtained from this study demonstrated that usingrhodamine 123 for container closure integrity testing with in syringe fluorescence measurements significantlyenhanced the sensitivity and robustness of the testing and effectively overcame limitations of the traditional methylene bluemethod with visual or UV-visible absorption detection.
 
Publisher PURKH
 
Date 2020-06-13
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://purkh.com/index.php/tochem/article/view/756
 
Source To Chemistry Journal; Vol. 6: May - August 2020; 48-54
2581-7507
 
Language eng
 
Relation https://purkh.com/index.php/tochem/article/view/756/730
 
Rights Copyright (c) 2020 Shripad Deshpande, Deepak D. Kayande, Mazahar Farooqui, Parag Deshmukh
https://creativecommons.org/licenses/by/4.0
 

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