A single Legionella pneumophila genotype in the freshwater system in a ship experiencing three separate outbreaks of legionellosis in six years

Microbial Ecology in Health and Disease

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Title A single Legionella pneumophila genotype in the freshwater system in a ship experiencing three separate outbreaks of legionellosis in six years
 
Creator Ahlen, Catrine; Norwegian Centre for Maritime Medicine, NSMM
Aas, Marianne; SINTEF, Trondheim
Krusnell, Jadwiga; Department of Microbiology, Parasitology and Waterborne Pathogens, Public Health Agency of Sweden, Stockholm
Iversen, Ole-Jan; Department of Laboratory Medicine, Children’s and Women’s Health, Faculty of Medicine, Norwegian University of Science and Technology, NTNU, Trondheim,
 
Subject Legionella pneumophila; Genotypes; Ships; infection outbreaks; Prevention and control; Pathogenicity; Virulence; Free-living amoeba
 
Description Background: Recurrent legionella outbreaks at one and the same location are common. We have identified a single Legionella pneumophila genotype associated with recurrent Legionella outbreaks over 6 years.Methods: Field emergency surveys following Legionella outbreaks were performed on a vessel in 2008, 2009 and 2013. Water samples from both the distribution and technical parts of the potable water system were analyzed with respect to L. pneumophila [Real-Time PCR, cultivation, serotyping and genotyping (PFGE)] and free-living amoebae, (FLA).Results: Legionella pneumophila serogroup 1 was present in the ship’s potable water system during every outbreak. Genotyping of the 2008 survey material showed two separate PFGE genotypes while those in 2009 and 2013 demonstrated the presence of only one of the two genotypes. FLA with intracellular L. pneumophila of the same genotype were also detected. Analyses of the freshwater system on a ship following three separate Legionella outbreaks, for L. pneumophila and FLAs, revealed a single L. pneumophila genotype and FLA (Hartmanella).Conclusions: It is reasonable to assume that the L. pneumophila genotype detected in the freshwater system was the causal agent in the outbreaks onboard. Persistence of an apparently low-pathogenic L. pneumophila genotype and FLA in a potable water system represent a potential risk for recurrent outbreaks.Keywords: Legionella pneumophila; Genotypes; Ships; infection outbreaks; Prevention and control; Pathogenicity; Virulence; Free-living amoeba(Published: 10 August 2016)Citation: Microbial Ecology in Health & Disease 2016, 27: 31148 - http://dx.doi.org/10.3402/mehd.v27.31148
 
Publisher Microbial Ecology in Health and Disease
 
Contributor
 
Date 2016-08-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
text/html
application/epub+zip
application/xml
 
Identifier http://www.microbecolhealthdis.net/index.php/mehd/article/view/31148
10.3402/mehd.v27.31148
 
Source Microbial Ecology in Health and Disease; Vol 27 (2016)
1651-2235
 
Language eng
 
Relation http://www.microbecolhealthdis.net/index.php/mehd/article/view/31148/48191
http://www.microbecolhealthdis.net/index.php/mehd/article/view/31148/48192
http://www.microbecolhealthdis.net/index.php/mehd/article/view/31148/48193
http://www.microbecolhealthdis.net/index.php/mehd/article/view/31148/48194
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/31148/25990
 
Rights Copyright (c) 2016 Catrine Ahlen, Marianne Aas, Jadwiga Krusnell, Ole-Jan Iversen
http://creativecommons.org/licenses/by-nc/4.0
 

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