Regulatory effect of paraprobiotic Lactobacillus gasseri CP2305 on gut environment and function

Microbial Ecology in Health and Disease

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Title Regulatory effect of paraprobiotic Lactobacillus gasseri CP2305 on gut environment and function
 
Creator Sugawara, Tomonori; Asahi Group Holdings, LTD.
Sawada, Daisuke; Asahi Group Holdings, LTD.
Ishida, Yu; Asahi Group Holdings, LTD.
Aihara, Kotaro; Asahi Group Holdings, LTD.
Aoki, Yumeko
Takehara, Isao; Clinical Support Corporation
Takano, Kazuhiko; Medical Corporation Hokubukai Utsukushigaoka Hospital
Fujiwara, Shigeru; Asahi Group Holdings, LTD.
 
Subject abdominal symptoms; fecal habits; intraluminal environment; bacterial flora; heat-inactivated lactic acid-producing bacilli; parasympathetic activation
 
Description Background: Lactobacillus gasseri CP2305 (CP2305) is a strain of Lactobacillus isolated from a stool sample from a healthy adult that showed beneficial effects on health as a paraprobiotic. In a previous study, we demonstrated that CP2305-fermented heat-treated milk modified gut functions more than artificially acidified sour milk. Thus, the regulatory activity of the former beverage was attributed to the inactivated CP2305 cells.Objective: The aim of this study was to elucidate the contribution of non-viable paraprobiotic CP2305 cells to regulating human gut functions. We thus conducted a randomized, placebo-controlled, double-blinded parallel group trial.Design: The trial included 118 healthy participants with relatively low or high stool frequencies. The test beverage was prepared by adding 1×1010 washed, heat-treated, and dried CP2305 cells directly to the placebo beverage. The participants ingested a bottle of the assigned beverage daily for 3 weeks and answered daily questionnaires about defecation and quality of life. Fecal samples were collected and the fecal characteristics, microbial metabolite contents of the feces and composition of fecal microbiota were evaluated.Results: The number of evacuations and the scores for fecal odors were significantly improved in the group that consumed the CP2305-containing beverage compared with those of the group that consumed the placebo (p=0.035 and p=0.040, respectively). Regarding the fecal contents of microbial metabolites, the level of fecal p-cresol was significantly decreased in the CP2305 group relative to that of the placebo group (p=0.013). The Bifidobacterium content of the intestinal microbiota was significantly increased in the CP2305 group relative to that of the placebo group (p<0.008), whereas the content of Clostridium cluster IV was significantly decreased (p<0.003). The parasympathetic nerve activity of the autonomic nervous system became dominant and the total power of autonomic activity was elevated in the CP2305 group (p=0.0401 and p=0.011, respectively).Conclusions: The continuous ingestion of heat-treated CP2305 cells clearly affected intestinal functionality. This is the first report of sterilized Lactobacillus cells having a significant impact on the environment and functions of the intestinal tract. The observed effects might be due, at least in part, to the brain–gut interaction.Keywords: abdominal symptoms; defecation habits; intraluminal environment; bacterial flora; heat-inactivated lactic acid-producing bacilli; parasympathetic activation(Published: 14 March 2016)Citation: Microbial Ecology in Health & Disease 2016, 27: 30259 - http://dx.doi.org/10.3402/mehd.v27.30259
 
Publisher Microbial Ecology in Health and Disease
 
Contributor Asahi Group Holdings, Ltd.
Calpis Co. Ltd.
 
Date 2016-03-14
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
text/html
application/epub+zip
application/xml
 
Identifier http://www.microbecolhealthdis.net/index.php/mehd/article/view/30259
10.3402/mehd.v27.30259
 
Source Microbial Ecology in Health and Disease; Vol 27 (2016)
1651-2235
 
Language eng
 
Relation http://www.microbecolhealthdis.net/index.php/mehd/article/view/30259/45538
http://www.microbecolhealthdis.net/index.php/mehd/article/view/30259/45539
http://www.microbecolhealthdis.net/index.php/mehd/article/view/30259/45540
http://www.microbecolhealthdis.net/index.php/mehd/article/view/30259/45541
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http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/30259/24201
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http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/30259/26123
 
Rights Copyright (c) 2016 Tomonori Sugawara, Daisuke Sawada, Yu Ishida, Kotaro Aihara, Isao Takehara, Kazuhiko Takano, Shigeru Fujiwara
http://creativecommons.org/licenses/by-nc/4.0
 

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