Host microbiota modulates development of social preference in mice

Microbial Ecology in Health and Disease

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Field Value
 
Title Host microbiota modulates development of social preference in mice
 
Creator Arentsen, Tim; Karolinska Institutet
Raith, Henrike
Qian, Yu
Forssberg, Hans
Diaz Heijtz, Rochellys
 
Subject brain development; germ-free mice; gene expression; synaptic plasticity genes; amygdala; BDNF
 
Description Background: Mounting evidence indicates that the indigenous gut microbiota exerts long-lasting programming effects on brain function and behaviour.Objective: In this study, we used the germ-free (GF) mouse model, devoid of any microbiota throughout development, to assess the influence of the indigenous microbiota on social preference and repetitive behaviours (e.g. self-grooming).Methods and results: Using the three-chambered social approach task, we demonstrate that when adult GF mice were given a choice to spend time with a novel mouse or object, they spent significantly more time sniffing and interacting with the stimulus mouse compared to conventionally raised mice (specific pathogen-free, SPF). Time spent in repetitive self-grooming behaviour, however, did not differ between GF and SPF mice. Real-time PCR–based gene expression analysis of the amygdala, a key region that is part of the social brain network, revealed a significant reduction in the mRNA levels of total brain-derived neurotrophic factor (BDNF), BDNF exon I-, IV-, VI-, IX-containing transcripts, and NGFI-A (a signalling molecule downstream of BDNF) in GF mice compared to SPF mice.Conclusion: These results suggest that differential regulation of BDNF exon transcripts in the amygdala by the indigenous microbes may contribute to the altered social development of GF mice.Keywords: brain development; germ-free mice; gene expression; synaptic plasticity genes; amygdala; BDNF(Published: 15 December 2015)Citation: Microbial Ecology in Health & Disease 2015, 26: 29719 - http://dx.doi.org/10.3402/mehd.v26.29719
 
Publisher Microbial Ecology in Health and Disease
 
Contributor Foundation Olle Engkvist Byggmästare
Swedish Brain Foundation
Swedish Research Council
Strategic Neuroscience Programme at Karolinska Institutet.
 
Date 2015-12-15
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
text/html
application/epub+zip
application/xml
 
Identifier http://www.microbecolhealthdis.net/index.php/mehd/article/view/29719
10.3402/mehd.v26.29719
 
Source Microbial Ecology in Health and Disease; Vol 26 (2015)
1651-2235
 
Language eng
 
Relation http://www.microbecolhealthdis.net/index.php/mehd/article/view/29719/44055
http://www.microbecolhealthdis.net/index.php/mehd/article/view/29719/44056
http://www.microbecolhealthdis.net/index.php/mehd/article/view/29719/44057
http://www.microbecolhealthdis.net/index.php/mehd/article/view/29719/44058
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/29719/23129
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/29719/23130
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/29719/24624
 
Rights Copyright (c) 2015 Rochellys Diaz Heijtz
http://creativecommons.org/licenses/by-nc/4.0
 

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