Characterisation of probiotic properties in human vaginal lactobacilli strains

Microbial Ecology in Health and Disease

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Title Characterisation of probiotic properties in human vaginal lactobacilli strains
 
Creator Hütt, Pirje
Lapp, Eleri
Štšepetova, Jelena
Smidt, Imbi
Taelma, Heleri
Borovkova, Natalja
Oopkaup, Helen
Ahelik, Ave
Rööp, Tiiu
Hoidmets, Dagmar
Samuel, Külli
Salumets, Andreas
Mändar, Reet
 
Subject vaginal lactobacilli; probiotic; Lactobacillus crispatus; Lactobacillus gasseri; Lactobacillus jensenii
 
Description Background: Vaginal lactobacilli offer protection against recurrent urinary infections, bacterial vaginosis, and vaginal candidiasis.Objective: To characterise the isolated vaginal lactobacilli strains for their probiotic properties and to compare their probiotic potential.Methods: The Lactobacillus strains were isolated from vaginal samples by conventional culturing and identified by sequencing of the 16S rDNA fragment. Several functional properties were detected (production of hydrogen peroxide and lactic acid; antagonistic activity against Escherichia coli, Candida albicans, Candida glabrata, and Gardnerella vaginalis; auto-aggregation and adhesiveness) as well as safety (haemolytic activity, antibiotic susceptibility, presence of transferrable resistance genes).Results: A total of 135 vaginal lactobacilli strains of three species, Lactobacillus crispatus (56%), Lactobacillus jensenii (26%), and Lactobacillus gasseri (18%) were characterised using several functional and safety tests. Most of L. crispatus (89%) and L. jensenii (86%) strains produced H2O2. The best lactic acid producers were L. gasseri (18.2±2.2 mg/ml) compared to L. crispatus (15.6±2.8 mg/ml) and L. jensenii (11.6±2.6 mg/ml) (p<0.0001; p<0.0001, respectively). L. crispatus strains showed significantly higher anti-E. coli activity compared to L. jensenii. L. gasseri strains expressed significantly lower anticandidal activity compared to L. crispatus and L. jensenii (p<0.0001). There was no significant difference between the species in antagonistic activity against G. vaginalis. Nearly a third of the strains were able to auto-aggregate while all the tested strains showed a good ability to adhere to HeLa cells. None of the tested lactobacilli caused haemolysis. Although phenotypical resistance was not found to ampicillin, chloramphenicol, erythromycin, gentamycin, tetracycline, and vancomycin, the erm(B), tet(M), and tet(K) were detected in some strains. All strains were resistant to metronidazole, trimethoprim/sulfamethoxazole, and kanamycin.Conclusions: Our study revealed that the production of different antimicrobial metabolites is highly strain-specific and that the metabolites are not correlated with each other. L. crispatus displays better antagonistic activity against E. coli and Candida spp. than L. gasseri and L. jensenii; therefore; a potential probiotic candidate could be found among L. crispatus strains.
 
Publisher Microbial Ecology in Health and Disease
 
Contributor
 
Date 2016-08-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
text/html
application/epub+zip
application/xml
 
Identifier http://www.microbecolhealthdis.net/index.php/mehd/article/view/30484
10.3402/mehd.v27.30484
 
Source Microbial Ecology in Health and Disease; Vol 27 (2016)
1651-2235
 
Language eng
 
Relation http://www.microbecolhealthdis.net/index.php/mehd/article/view/30484/48217
http://www.microbecolhealthdis.net/index.php/mehd/article/view/30484/48218
http://www.microbecolhealthdis.net/index.php/mehd/article/view/30484/48219
http://www.microbecolhealthdis.net/index.php/mehd/article/view/30484/48220
http://www.microbecolhealthdis.net/index.php/mehd/article/downloadSuppFile/30484/24717
 
Rights Copyright (c) 2016 Pirje Hütt
http://creativecommons.org/licenses/by-nc/4.0
 

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