Human brucellosis among pyrexia of unknown origin cases and occupationally exposed individuals in Goa Region, India

Emerging Health Threats Journal

View Publication Info
Field Value
Title Human brucellosis among pyrexia of unknown origin cases and occupationally exposed individuals in Goa Region, India
Creator Pathak, Ajay D.
Dubal, Zunjar B.
Doijad, Swapnil P.
Raorane, Abhay
Rodrigues, Savio
Naik, Rajeshwar
Naik-Gaonkar, Shraddha
Kalorey, Dewanand R.
Kurkure, Nitin V.
Naik, Rajesh
Barbuddhe, Sukhadeo B.; Indian Council of Agricultural Research
Subject Epidemiology,; Infectious Disease; Serology
brucellosis; human; India; serology; ELISA; culture; PCR

Description Background: Brucellosis is a widespread zoonotic infection. This disease is endemic in many parts of Asia, including India. Brucellosis is a major cause of pyrexia of unknown origin (PUO). Persons exposed to infected animals or contaminated animal products are at high risk. Seropositivity among animal handlers, veterinarians and dairy workers has been documented in India. Thus, the present study was aimed to determine prevalence of brucellosis among PUO cases and occupationally exposed individuals.Methods: In this study, serum samples (n=282) from cases of pyrexia of unknown origin (PUO) (n=243), and occupationally exposed individuals (n=39) were collected and tested for brucellosis by Rose Bengal plate test (RBPT), serum agglutination test (SAT), indirect ELISA, IgG and IgM ELISA. Blood culture for isolation of Brucella was performed for 10 serologically positive patients using BACTEC 9050 automated blood culture system. Biochemical tests and PCR techniques were used for confirmation of the isolates.Results: Of the samples tested, 4.25%, 3.54%, 6.02% and 4.96% samples were positive by RBPT, SAT, indirect ELISA and IgG ELISA, respectively. None of the sample was positive for IgM ELISA. Of the 10 blood samples cultured bacteriologically, one Brucella isolate was recovered. The isolate was confirmed as Brucella abortus. Amplification of the bcsp31 and IS711 genes was also observed.Conclusion: Seropositivity for brucellosis was observed among PUO cases, animal handlers and dairy workers in Goa, India. The serological tests showed variable results. One Brucella isolate was obtained by performing blood culture. Confirmation of the case was done rapidly using molecular tools. General awareness about clinical symptoms should be increased which will improve proper diagnosis within short time frame.Keywords: brucellosis; human; India; serology; ELISA; culture; PCR(Published: 22 April 2014)Citation: Emerg Health Threats J 2014, 7: 23846 -
Publisher Emerging Health Threats Journal
Date 2014-04-22
Type info:eu-repo/semantics/article

Format application/pdf
Source Emerging Health Threats Journal; Vol 7 (2014) incl Supplements
Language eng
Coverage Western India


Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us


Copyright © 2015-2018 Simon Fraser University Library