Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress

Jurnal Pendidikan Kimia

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Title Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress
 
Creator Silaban, Saronom
Simorangkir, Murniaty
Gaffar, Shabarni
Maksum, Iman Permana
Subroto, Toto
 
Subject Kimia Terapan
 
Description Many proteins produced in E. coli accumulate in inclusion bodies. This study aims to detect the role of temperature in reducing the formation of inclusion bodies during recombinant human prethrombin-2expressed in E. coli BL21 (DE3) Arctic Express host. In this study, we created a host growth curve to find out the right time to add an inducer. The inducer used in our experiment was IPTG 0.1 mM. The fermentation process use a temperature of 12°C and 22°C. The results showed that recombinant human prethrombin-2 was successfully expressed as protein soluble using an optimum temperature of 12°C in E. coli BL21 (DE3) Arctic Express. It was indicated from the 63kDa protein band obtained from the soluble fraction on SDS-PAGE. The higher temperature of fermentation increased the amount of protein in the insoluble fraction due. It concluded that the fermentation temperature affect the rate of prethrombin-2 expression.Keywords: E. coli BL21(DE3) ArcticExpress, prethrombin-2, soluble, temperature
 
Publisher Jurnal Pendidikan Kimia
 
Contributor
 
Date 2019-12-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://jurnal.unimed.ac.id/2012/index.php/jpk/article/view/15779
10.24114/jpkim.v11i3.15779
 
Source Jurnal Pendidikan Kimia; Vol 11, No 3 (2019): December 2019; 122-128
2549-3116
2085-3653
 
Language eng
 
Relation https://jurnal.unimed.ac.id/2012/index.php/jpk/article/view/15779/12417
 
Rights Copyright (c) 2019 JURNAL PENDIDIKAN KIMIA
 

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