Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.)

AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian

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Title Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.)
Comparative Analysis on Metabolites of Torbangun (Plectranthus amboinicus (Lour.) Spreng.) Leaves Mutant
 
Creator Aisyah, Syarifah Iis
Rusmiyati, Henny
Sukma, Dewi
Damanik, Rizal
Nurcholis, Waras
 
Description Torbangun (Plectranthus amboinicus) merupakan tanaman obat dari keluarga Lamiaceae. Pada masyarakat Batak, daun torbangun digunakan untuk meningkatkan produksi air susu. Keragaman kandungan fitokimia dapat ditingkatkan salah satunya dengan teknik iradiasi sinar gamma untuk program pemuliaan tanaman. Pada penelitian ini, metode metabolomik yang tidak tertarget digunakan untuk mengevaluasi profil senyawa pada bagian daun tanaman mutan dari iradiasi sinar gamma dan kontrol. Kandungan senyawa planlet mutan dan kontrol dianalisis dengan GC-MS. Data GC-MS dianalisis dengan kemometrik dengan menggunakan hierarchical cluster analysis (HCA). Keragaman kandungan kimia ditunjukkan oleh planlet mutan dan kontrol daun torbangun. Hasil analisa planlet kontrol torbangun menunjukkan terdapat lima senyawa utama diantaranya hydroxymethylfurfurole (35,15%), brevifolin (7,69%), 3-Dihydro-3,5-Dihydroxy-6-Methyl-4H-Pyran-4-one (DDMP) (13,13%), stigmasterol (6,51%) dan ferruginol (8.63%). Hasil analisa mutan torbangun memiliki kandungan senyawa DDMP (19,57%), neophytadiene (8,47%), linolenic acid (9,10%), ferruginol (7,61%), stigmasterol (14,14%) dan gamma.-sitosterol (7,08%). HCA menunjukkan tiga komponen senyawa yang berbeda antara planlet kontrol dan mutan yaitu kenaikan kandungan senyawa DDMP dan stigmasterol, serta penurunan kandungan hydroxymethylfurfurole pada mutan torbangun.
Torbangun (Plectranthus amboinicus) is a medicinal plant belonging to the Lamiaceae family. In Batak society, the torbangun leaves are consumed to increase breast milk production. The phytochemical variability can be improved by gamma-ray irradiation for plant breeding programs. In this work, a non-targeted metabolomics method was followed to evaluate the compounds profile of irradiated mutant and wild-type on leaves of P. amboinicus. GC-MS analyses evaluated the mutant putative (derived from gamma-ray irradiation) and wild-type plantlets. The resulting of GC-MS data were analyzed with chemometric analysis using hierarchical cluster analysis (HCA). The chemical diversity was showed in mutant and wild-type P. amboinicus. The major metabolite in plantlet wild-type were hydroxymethylfurfural (35.15%), brevifolin (7.69%), 3-Dihydro-3,5-Dihydroxy-6-Methyl-4H-Pyran-4-one (DDMP) (13.13%), stigmasterol (6.51%) and ferruginol (8.63%). However, the compound of DDMP (19.57%), neophytadiene (8.47%), linolenic acid (9.10%), ferruginol (7.61%), stigmasterol (14.14%) and gamma-sitosterol (7.08%) were identified in mutant plantlet. HCA showed diversity between wild-type and mutant plantlets, which highest content of DDMP and stigmasterol and lowest of hydroxymethylfurfural of mutant plantlet.
 
Publisher Universitas Bangka Belitung
 
Date 2020-03-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://agrosainstek.ubb.ac.id/index.php/agrosainstek/article/view/109
10.33019/agrosainstek.v4i1.109
 
Source AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian; Vol 4 No 1 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian; 10-16
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian; Vol 4 No 1 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian; 10-16
2579-843X
2615-2207
10.33019/agrosainstek.v4i1
 
Language eng
 
Relation https://agrosainstek.ubb.ac.id/index.php/agrosainstek/article/view/109/40
 
Rights Copyright (c) 2020 Syarifah Iis Aisyah, Henny Rusmiyati, Dewi Sukma, Rizal Damanik, Waras Nurcholis
http://creativecommons.org/licenses/by/4.0
 

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