Isolation, Screening and Characterization of Exopolysaccharide Producing Bacteria

Microbiology Research Journal International

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Title Isolation, Screening and Characterization of Exopolysaccharide Producing Bacteria
Creator Nwosu, I. G.
Abu, G. O.
Agwa, K. O.
Description Exopolysaccharides(EPSs) are essential metabolites synthesized and excreted by certain microorganisms in response to extreme condition of pH, temperature, salinity, osmotic stress and other contaminants for survival in such adverse environment. The present study focuses on isolation of exopolysaccharide producing bacteria from extreme environment of oil polluted soil of Ogoni land and marine water of Bonny Island. Screening of EPS producing abilities of the selected isolates were estimated using two approaches viz gravimetric analysis of EPS dry weight and quantification assay for total carbohydrate content by phenol sulphuric acid method. Result revealed that a total of forty (40) different colonies were suspected to produce exopolysaccharide after preliminary screening by selecting thick ropy-like colony formers on agar medium. Secondary screening indicated that twelve (12) isolates produced precipitates above 1000mg/l of total dry weight and eight (8) isolates produced highest EPS yield above 1000mg/l of culture media with carbohydrate content determination. Five isolates with the code WAS1, WAS11, SC6, SOS7 and SOS10 produced significantly higher EPS compared to other bacterial colonies isolated and were termed as most potent EPS producers. These isolates were identified based on 16S rDNA sequence as Providencia stuarti, Escherichia coli, Shewanella chilikensis and Bacillus nealsonii. The result of the present study indicated that these strains have the potentials of producing high exopolysaccharides and can be explored in biotechnological industries.
Publisher SCIENCEDOMAIN international
Date 2019-11-16
Type info:eu-repo/semantics/article
Format application/pdf
Source Microbiology Research Journal International; 2019 - Volume 29 [Issue 5]; 1-9
Language eng
Rights Copyright (c) 2019 © 2019 Nwosu et al.; This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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