Antimicrobial activity of endophytic fungi from the medicinal plants Mammea americana (Calophyllaceae) and Moringa oleifera (Moringaceae)

Biomédica

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Title Antimicrobial activity of endophytic fungi from the medicinal plants Mammea americana (Calophyllaceae) and Moringa oleifera (Moringaceae)
Actividad antimicrobiana de hongos endófitos de las plantas medicinales Mammea americana (Calophyllaceae) y Moringa oleifera (Moringaceae)
 
Creator Mosquera, Wilmer Giovanny
Criado , Libeth Yajaira
Guerra, Beatriz Elena
 
Subject Drug resistance, microbial
endophytes
plants, medicinal
Escherichia coli
Staphylococcus aureus
farmacorresistencia microbiana
endófitos
plantas medicinales
Escherichia coli
Staphylococcus aureus
 
Description Introduction: Infectious diseases represent one of the leading causes of death worldwide. Considering the growing global challenge of antimicrobial resistance, research into new sources of potentially effective antimicrobial agents from natural origins is of great importance for world health.Objective: To evaluate the antimicrobial activity of endophytic fungi from Mammea americana and Moringa oleifera upon Staphylococcus aureus (ATCC 29213), S. aureus (resistant strain USb003), Escherichia coli (ATCC 25922), and E. coli (resistant strain USb007).Materials and methods: We isolated endophytic fungi from the leaves, seeds, and stems of the two plants under study. We evaluated their antimicrobial activity through the formation of sensitivity haloes in dual tests in vitro, as well as in trials using crude ethanolic extracts from the endophytes. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and cytotoxicity o the substances were analyzed.Results: Three ethanolic extracts of Penicillium sp., Cladosporium (001), and Cladosporium (002) exhibited the greatest inhibition halos in sensitive and resistant strains of E. coli and S. aureus. The MIC and CBM found were statistically significant (p≤0.05) compared with the gentamicin control. Furthermore, the cytotoxicity test results of CC50>1,000 demonstrated that the endophytic fungi studied exhibit bactericidal characteristics without causing unintended damage.Conclusion: The endophytic fungi M. oleifera and M. americana represent a source of active secondary metabolites with antimicrobial and non-toxic properties. In light of these findings, further research should proceed with chemical identification of the compounds and the study of their mechanisms of action, especially given the paucity of current scientific knowledge concerning the isolation of endophytes in these plants.
Introducción. Las enfermedades infecciosas son una causa importante de muertes en el mundo. La resistencia antimicrobiana es un problema global, por lo que es conveniente la investigación de nuevas fuentes de agentes antimicrobianos de origen natural potencialmente efectivos.Objetivo. Evaluar la actividad antimicrobiana de hongos endófitos de Mammea americana y Moringa oleifera en la cepa sensible (ATCC 29213) y en la cepa resistente (USb003) de Staphylococcus aureus, así como en la cepa sensible (ATCC 25922) y la cepa resistente (USb007) de Escherichia coli.Materiales y métodos. Se aislaron 14 hongos endófitos de las hojas, semillas y tallos de las dos plantas en estudio. Se evaluó su actividad antimicrobiana mediante la formación de halos de sensibilidad por ensayo dual in vitro y pruebas con extractos etanólicos crudos provenientes de los endófitos a los que se les evaluó la concentración mínima inhibitoria (CMI), la concentración bactericida mínima (CBM) y la citotoxicidad.Resultados. Tres extractos etanólicos de Penicillium sp., Cladosporium sp. (001) y Cladosporium sp. (002) presentaron mayores halos de inhibición en cepas sensibles y resistentes de E. coli y S. aureus. La CMI y la CBM halladas fueron estadísticamente significativas (p≤0,05), comparadas con el control de gentamicina. Las pruebas de citotoxicidad (concentración citotóxica, CC50>1.000) demostraron que los hongos endófitos poseen características bactericidas y no ocasionan daño alguno.Conclusión. Se halló una fuente de metabolitos secundarios activos con propiedades antimicrobianas y no tóxicas en los hongos endófitos de M. oleifera y M. americana; estos hallazgos son importantes para continuar con la identificación química de los compuestos y el estudio de sus mecanismos de acción en estas plantas en las que el aislamiento de endófitos ha sido escaso.
 
Publisher Instituto Nacional de Salud
 
Date 2020-03-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
text/xml
 
Identifier https://revistabiomedica.org/index.php/biomedica/article/view/4644
10.7705/biomedica.4644
 
Source Biomedica; Vol. 40 No. 1 (2020); 55-71
Biomédica; Vol. 40 Núm. 1 (2020); 55-71
2590-7379
0120-4157
 
Language spa
 
Relation https://revistabiomedica.org/index.php/biomedica/article/view/4644/4351
https://revistabiomedica.org/index.php/biomedica/article/view/4644/4539
/*ref*/von Wintersdorff CJH, Penders J, van Niekerk JM, Mills ND, Majumder S, van Alphen LB, et al. Dissemination of antimicrobial resistance in microbial ecosystems through horizontal gene transfer. Front Microbiol. 2016;7:173. https://doi.org/10.3389/fmicb.2016.00173 2. Bisht D, Owais M, Venkatesan K. Potential of plant‐derived products in the treatment of mycobacterial infections. En: Ahmad I, Aqil F, Owais M, editors. Modern phytomedicine: Turning medicinal plants into drugs. Weinheim: Wiley-VCH Verlag GmbH & Co. KGaA; 2006. p. 295-6. https://doi.org/10.1002/9783527609987.ch14 3. Alvin A, Miller KI, Neilan BA. Exploring the potential of endophytes from medicinal plants as sources of antimycobacterial compounds. Microbiol Res. 2014;169:483-95. https://doi.org/10.1016/j.micres.2013.12.009 4. Bary A. Morphologie und Physiologie der Pilze, Flechten und Myxomyceten. W. Leipzig : W. Engelmann; 1866. p. 21-2. https://doi.org/10.5962/bhl.title.120970 5. Wilson D. Endophyte: The evolution of a term, and clarification of its use and definition. Oikos.1995;73:274-6. https://doi.org/10.2307/3545919 6. Schulz BJ, Boyle C. The endophytic continuum review. Mycol Res. 2005;109:661-86. https://doi.org/10.1017/S095375620500273X 7. Kusari S, Hertweck C, Spiteller M. Chemical ecology of endophytic fungi: Origins of secondary metabolites. Chem Biol. 2012;19:792-8. https://doi.org/10.1016/j.chembiol.2012.06.004 8. Heinig U, Scholz S, Jennewein S. Getting to the bottom of Taxol biosynthesis by fungi. Fungal Divers. 2013;60:161-70. https://doi.org/10.1007/s13225-013-0228-7 9. Guo B, Dai JR, Ng S, Huang Y, Leong C, Ong W, Carté BK. Cytonic acids A and B: Novel tridepside inhibitors of hCMV protease from the endophytic fungus Cytonaema species. J Nat Prod. 2000;63:602-4. https://doi.org/10.1021/np990467r 10. Mahecha VG, Ovalle EA, Camelo SD, Rozo FA, Barrero BD. Vegetación del territorio CAR: 450 especies de sus llanuras y montañas. Fecha de consulta: 18 de noviembre de 2017. Disponible en: http://biblioteca.humboldt.org.co/cgi-bin/koha/opac-detail.pl?biblionumber=5243&query_desc=au%2Cwrdl%3A%20Barrero%20 11. Little EL, Woodbury R, Wadsworth FH. Trees of Puerto Rico and the Virgin Islands. Fecha de consulta: 20 de noviembre de 2017. Disponible en: http://edicionesdigitales.info/biblioteca/arbolesprvi2engl.pdf 12. Yang H, Jiang B, Reynertson KA, Basile MJ, Kennelly EJ. Comparative analyses of bioactive Mammea Coumarins from seven parts of Mammea americana by HPLC-PDA with LC-MS. J Agric Food Chem. 2006;54:4114-20. https:/doi.org/10.1021/jf0532462 13. Sánchez-Peña YA, Martínez-Ávila GC, Sinagawa-García SR, Vázquez-Rodríguez JA. Moringa oleifera; importancia, funcionalidad y estudios involucrados. Revista Científica de la Universidad Autónoma de Coahuila. 2013;5:25-30. 14. Fischer HW. Moringa oleifera. Magic, myth or miracle. Salem-USA: Britannia Printing; 2012. p. 50-136. 15. Alfaro N, Martínez W. Uso potencial de la Moringa (Moringa oleifera Lam) para la producción de alimentos nutricionalmente mejorados. Fonacyt. Fecha de consulta: 18 de noviembre de 2017. Disponible en: https://docplayer.es/89330773-Uso-potencial-de-la-moringa-moringaoleifera-lam-para-la-produccion-de-alimentos-nutricionalmente-mejorados.html 16. Souza IF, Napoleão TH, de Sena KX, Paiva PM, de Araújo JM, Coelho LC. Endophytic microorganisms in leaves of Moringa oleifera collected in three localities at Pernambuco State, Northeastern Brazil. Microbiology Research Journal International. 2016;3:1-7 https://doi.org/10.9734/BMRJ/2016/24722 17. Zhao Z, Wang Q, Wang K, Brian K, Liu C, Gu Y. Study of the antifungal activity of Bacillus vallismortis ZZ185 in vitro and identification of its antifungal components. Bioresour Technol. 2010;101:292-7. https://doi.org/10.1016/j.biortech.2009.07.071 18. Echavarría A, D’ Armas H, Matute NL, Jaramillo C, Rojas-de-Astudillo L, Benítez R. Evaluation of antioxidant capacity and secondary metabolites of sixteen medicinal plants extracts. Ciencia Unemi. 2016;9:29-35. 19. Ahmedkhan H, Ahmad A, Mehboob R. Nosocomial infections and their control strategies. Asian Pac J Trop Biomed. 2015; 5509-14. https://doi.org/10.1016/j.apjtb.2015.05.001 20. Blanco J, Blanco M, Blanco JE, Mora A, Alonso MP, González EA, et al. Enterobacterias: características generales. Género Escherichia. En: Vadillo S, Piriz S, Mateos E, editores. Manual de Microbiología Veterinaria. Madrid: McGraw-Hill Interamericana; 2002. p. 301-25. 21. de la Hoz FJ, Santiago L. Infección urinaria recurrente en la mujer posmenopáusica. Revista Colombiana de Menopausia. 2018:19:3-4. 22. Pigrau C. Infecciones del tracto urinario nosocomiales. Enferm Infect Microbiol Clin. 2013;31:614-24. https://doi.org/10.1016/j.eimc.2012.11.015 23. Schulz B, Boyle C. The endophytic continuum. Mycol Res. 2005;109:661-86. https://doi.org/10.1017/S095375620500273X 24. Olaechea PM, Insaustib J, Blanco A, Luque P. Epidemiología e impacto de las infecciones nosocomiales. Medicina Intensiva. 2010;34:256-67. https://doi.org/10.1016/j.medin.2009.11.013 25. Gil de MM. Staphylococcus aureus: microbiología y aspectos moleculares de la resistencia a meticilina. Rev Chil Infectol. 2000;17:145-52. https://doi.org/10.4067/S0716-10182000000200010 26. Carroll K, Hobden J.A, Miller S, Morse S, Mietzner T, Detrick B, et al. Jawetz Melnick & Adelbergs Medical Microbiology. 27th edition. New York: Mc Graw Hill Education; 2016. p. 363-94. 27. World Health Organization. Global strategy for containment of antimicrobial resistance. Geneve: WHO; 2018. p. 3-4. https://www.who.int/es/news-room/fact-sheets/detail/resistencia-a-los-antimicrobianos 28. Unterseher M, Schnittler M. Dilution-to-extinction cultivation of leaf-inhabiting endophytic fungi in beech (Fagus sylvatica L.) –different cultivation techniques influence fungal biodiversity assessment. Mycol Res. 2009;113:645-54. https://doi.org/10.1016/j.mycres.2009.02.002 29. Carbungco ES, Pedroche NB, Panes VA, De la Cruz TE. Identification and characterization of endophytic fungi associated with the leaves of Moringa oleifera Lam. Acta Horticulturae. 2017;1158:373-80. https://doi.org/10.17660/ActaHortic.2017.1158.42 30. Watanabe T. Pictorial Atlas of Soil and Seed Fungi: Morphologies of cultured fungi and key to species. Boca Raton: CRC Press Taylor & Francis Group; 2010. p. 426. 31. Mishra VK, Passari AK, Chandra P, Leo VV, Kumar B, Uthandi S, et al. Determination and production of antimicrobial compounds by Aspergillus clavatonanicus strain MJ31, an endophytic fungus from Mirabilis jalapa L. using UPLC-ESI-MS/MS and TD-GC-MS analysis. PloS One. 2017;12:e0186234. https://doi.org/10.1371/journal.pone.0186234 32. Liang H, Xing Y, Chen J, Zhang D, Guo S, Wang C. Antimicrobial activities of endophytic fungi isolated from Ophiopogon japonicus (Liliaceae). BMC Complement Altern Med. 2012;12:2-6. https://doi.org/10.1186/1472-6882-12-238 33. Dos Santos IP, da Silva LC, da Silva MV, de Araújo JM, Cavalcanti Mda S, Lima VL. Antibacterial activity of endophytic fungi from leaves of Indigofera suffruticosa Miller (Fabaceae). Front Microbiol. 2015;350:1-7. https://doi.org/10.3389/fmicb.2015.00350 34. Zhao J, Shan T, Mou Y, Zhou L. Plant-derived bioactive compounds produced by endophytic fungi. Mini Rev Med Chem. 2011;11:159-68. https://doi.org/10.2174/138955711794519492 35. Gond SK, Mishra A, Sharma VK, Verma SK, Kumar J, Kharwar RN, et al. Diversity and antimicrobial activity of endophytic fungi from isolated from Nyctanthes arbor-tristis, a well know medicinal plant of India. Mycoscience. 2011;53:113-21. https://doi.org/10.1007/S10267-011-0146-Z 36. Yu H, Zhang L, Li L, Zheng C, Guo L, Li W, et.al. Recent developments and future prospects of antimicrobial metabolites produced by endophytes. Microbiol Res. 2010;165:437-49. https://doi.org/10.1016/j.micres.2009.11.009 37. Idris A, Al-tahir L, Idris E. Antibacterial activity of endophytic fungi extracts from the medicinal plant Kigelia africana. Egypt Acad J Biolog Sci. 2013;5:1-9. 38. Rajeswari S, Umamaheswari S, Prasanth D.A, Rajamanikandan KC. Study of endophytic fungal community of Moringa oleifera from Omalur Region – Salem. Int J Pharm Sci Res. 2014;5:4887-92. https://doi.org/10.13040/IJPSR.0975-8232.5(11).4887-92 39. Sugijanto EN, Dorra LB. Antimicrobial activity of Cladosporium oxysporum endophytic fungus extract isolated from Aglaia odorata Lour. Indonesian Journal of Medicine. 2016;2:108-15. https://doi.org/10.26911/theijmed.2016.01.02.04 40. de Medeiros LS, Murgu M, de Souza AQ, Rodrigues FO. Antimicrobial depsides produced by Cladosporium uredinicola, an endophytic fungus isolated from Psidium guajava fruits. Helv Chim Acta. 2011;94:1077-84 https://doi.org/10.1002/hlca.201000387 41. Prihanto A, Firdaus M, Nurdiani R. Endophytic fungi isolated from Mangrove (Rhyzopora mucronata) and its antibacterial activity on Staphylococcus aureus and Escherichia coli. J Food Sci Eng. 2011;1:386-9 42. Dhanalakshmi R, Umamaheswari S, Sugandhi P, Prasanth DA. Biodiversity of the endophytic fungi isolated from Moringa oleifera of Yercaud hills. Int J Pharm Sci Res. 2013;4:1064-8. https://doi.org/10.13040/IJPSR.0975-8232.4(3).1064-68
 
Rights Derechos de autor 2020 Biomédica
https://creativecommons.org/licenses/by/4.0
 

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